Porous zirconia particles, and aggregate for immobilizing protein
Porous zirconia particles exhibit high specificity to a protein to be immobilized thereto and are used in immobilization of the protein. The porous zirconia particles have a pore diameter D50, at which a ratio of a cumulative pore volume to a total pore volume is 50%, the pore diameter D50 being in a range of 3.20 nm or more and 6.50 nm or less; and a pore diameter D90, at which a ratio of a cumulative pore volume to a total pore volume is 90%, the pore diameter D90 being in a range of 10.50 nm or more and 100.00 nm or less. The total pore volume of the particles is greater than 0.10 cm 3 /g. D50, D90, and the total pore volume are determined based on a pore diameter distribution measured through a BET method.
1. Porous zirconia particles for immobilization of a protein, characterized in that the porous zirconia particles have:
a pore diameter D50, at which a ratio of a cumulative pore volume to a total pore volume is 50%, is in a range of 3.20 nm or more and 6.50 nm or less; and
a pore diameter D90, at which a ratio of a cumulative pore volume to the total pore volume is 90%, is in a range of 10.50 nm or more and 100.00 nm or less, wherein
the total pore volume is greater than 0.10 cm 3 /g, and
wherein the pore diameter D50, the pore diameter D90, and the total pore volume are determined based on a pore diameter distribution measured through a BET method.
2. The porous zirconia particles according to claim 1 , wherein the protein is immunoglobulin.
3. The porous zirconia particles according to claim 2 , wherein the immunoglobulin is at least one species selected from the group consisting of IgG, IgE, and IgD.
4. The porous zirconia particles according to claim 1 , wherein the porous zirconia particles have surfaces onto which a chelating agent is bound.