IMMUNOGLOBULIN PURIFICATION METHOD AND IMMUNOGLOBULIN PURIFICATION DEVICE, AND IMMUNOGLOBULIN PRODUCTION METHOD AND IMMUNOGLOBULIN PRODUCTION DEVICE
To provide an immunoglobulin purification method which achieves a high immunoglobulin recovery percentage without causing loss of the antibody nature of an immunoglobulin. The immunoglobulin purification method includes an adsorption step and a desorption step. The adsorption step involves adsorption of an immunoglobulin onto porous zirconia particles in a neutral buffer. The desorption step involves desorption of the immunoglobulin adsorbed on the porous zirconia particles from the porous zirconia particles by means of a neutral desorption liquid.
1 . An immunoglobulin purification method comprising the steps of:
adsorbing an immunoglobulin onto porous zirconia particles in a neutral buffer; and
desorbing the immunoglobulin adsorbed on the porous zirconia particles from the porous zirconia particles by means of a neutral desorption liquid.
2 . The immunoglobulin purification method according to claim 1 , wherein the desorption liquid is a phosphate buffer.
3 . The immunoglobulin purification method according to claim 1 , wherein the desorption liquid contains at least one species selected from the group consisting of a compound represented by the following formula (1):
(wherein R 1 represents a C1 to C10 alkylene group; and each of R 2 to R 5 , which may be identical to or different from one another, represents a C1 to C10 alkylene group) and a salt thereof.
4 . An immunoglobulin purification device comprising:
an adsorption unit that is configured to absorb an immunoglobulin onto porous zirconia particles in a neutral buffer; and
a desorption unit that is configured to desorb the immunoglobulin adsorbed on the porous zirconia particles from the porous zirconia particles by means of a neutral desorption liquid.
5 . The immunoglobulin purification device according to claim 4 , wherein the desorption liquid is a phosphate buffer.
6 . The immunoglobulin purification device according to 4, wherein the desorption liquid contains at least one species selected from the group consisting of a compound represented by formula (1):
(wherein R 1 represents a C1 to C10 alkylene group; and each of R 2 to R 5 , which may be identical to or different from one another, represents a C1 to C10 alkylene group) and a salt thereof.
7 . An immunoglobulin production method comprising the steps of:
adsorbing an immunoglobulin onto porous zirconia particles in a neutral buffer; and
desorbing the immunoglobulin adsorbed on the porous zirconia particles from the porous zirconia particles by means of a neutral desorption liquid.
8 . The immunoglobulin production method according to claim 7 , wherein the desorption liquid is a phosphate buffer.
9 . The immunoglobulin production method according to claim 7 , wherein the desorption liquid contains at least one species selected from the group consisting of a compound represented by formula (1):
(wherein R 1 represents a C1 to C10 alkylene group; and each of R 2 to R 5 , which may be identical to or different from one another, represents a C1 to C10 alkylene group) and a salt thereof.
10 . An immunoglobulin production device comprising:
an adsorption unit that is configured to absorb an immunoglobulin onto porous zirconia particles in a neutral buffer; and
a desorption unit that is configured to desorb the immunoglobulin adsorbed on the porous zirconia particles from the porous zirconia particles by means of a neutral desorption liquid.
11 . The immunoglobulin production device according to claim 10 , wherein the desorption liquid is a phosphate buffer.
12 . The immunoglobulin production device according to claim 10 , wherein the desorption liquid contains at least one species selected from the group consisting of a compound represented by formula (1):
(wherein R 1 represents a C1 to C10 alkylene group; and each of R 2 to R 5 , which may be identical to or different from one another, represents a C1 to C10 alkylene group) and a salt thereof.