Lecithin vesicles
Giant multi-lamellar vesicles (GMVs) comprising lecithin are provided which are about 3 to about 15 μm in size. Methods for preparing the GMVs, and for preparing large unilamellar vesicles (LUVs) from the GMVs, are provided, as well as methods for encapsulating cargo within the GMVs and LUVs. The present vesicles are useful for the oral delivery of encapsulated cargo.
1. Large unilamellar vesicles having a size in the range of about 100-400 nm consisting essentially of a combination and admixture of lecithin and a polyol in an aqueous buffer, wherein said vesicles have a water activity of less than 0.9.
2. The unilamellar vesicles of claim 1 , wherein the lecithin is deoiled lecithin.
3. The unilamellar vesicles of claim 1 , wherein the lecithin comprises phosphatidylcholines in an amount in the range of about 15-50% by wt, phosphatidylethanolamines in an amount in the range of about 5-25 wt % and phosphatidic acids in an amount of less than 10% by wt.
4. The unilamellar vesicles of claim 1 , wherein the polyol is glycerol.
5. The unilamellar vesicles of claim 1 , comprising cargo.
6. The unilamellar vesicles of claim 1 , having a water activity of less than 0.85.
7. The unilamellar vesicles of claim 5 , wherein the cargo is hydrophilic.
8. The unilamellar vesicles of claim 5 , wherein the cargo is hydrophobic.
9. A method of making large unilamellar vesicles as defined in claim 1 comprising the step of mixing without homogenization giant multi-lamellar vesicles (GMVs) in the size range of 3-10 μm consisting essentially of lecithin with a low molecular weight polyol in an aqueous buffer for a sufficient period of time.
10. The method of claim 9 , wherein the polyol is glycerol.
11. The method of claim 9 , wherein the polyol is used in an amount of 10-90% by wt.
12. The method of claim 9 , wherein the vesicles are reduced to a size within the range of 50 to 400 nm.
13. The method of claim 12 , wherein the vesicles are reduced to a size within the range of 50-150 nm.
14. The method of claim 9 , wherein the vesicles exhibit a water activity of less than 0.85.
15. The method of claim 9 , wherein the lecithin is deoiled lecithin.
16. The method of claim 9 , wherein the lecithin comprises phosphatidylcholines in an amount in the range of about 15-50% by wt, phosphatidylethanolamines in an amount in the range of about 5-25 wt % and phosphatidic acids in an amount of less than 10% by wt.
17. The method of claim 11 , wherein the polyol is used in an amount of greater than 30% by wt.
18. The method of claim 11 , wherein the polyol is glycerol and is used in an amount of 40-90% by wt.