Crystalline forms of 4-(7-hydroxy-2-isopropyl-4-oxo-4H-quinazolin-3-yl)-benzonitrile and formulations thereof
The present disclosure provides polymorphs and formulations of 4-(7-hydroxy-2-isopropyl-4-oxo-4H-quinazolin-3-yl)-benzonitrile (compound I). The present disclosure further provides methods for treating ocular surface pain by administering 4-(7-hydroxy-2-isopropyl-4-oxo-4H-quinazolin-3-yl)-benzonitrile (compound I). The present invention also provides methods for treating dry eye disease and ocular hyperemia by administering 4-(7-hydroxy-2-isopropyl-4-oxo-4H-quinazolin-3-yl)-benzonitrile.
1 . A crystal form A of 4-(7-Hydroxy-2-isopropyl-4-oxo-4H-quinazolin-3-yl)benzonitrile (compound I) having structure
characterized by an X ray diffraction pattern comprising peaks at 2θ values of 7.2, 12.7, and 21.4±0.2 °2θ.
2 . The crystal form A of compound I according to claim 1 , characterized by an X ray diffraction pattern comprising three or more peaks at 2θ values selected from 7.2, 12.7, 13.9, 18.1, 21.4, 25.1, and 26.8±0.2 °2θ.
3 . A method of preparing a crystal form A of compound I according to claim 1 , comprising cooling a hot solution of the free base of compound I in methanol and cooling to about 0° C., to crystallize compound I as crystal form A.
4 . A crystal form C of 4-(7-Hydroxy-2-isopropyl-4-oxo-4H-quinazolin-3-yl)benzonitrile (compound I) having structure
characterized by an X ray diffraction pattern comprising peaks at 2θ values of 7.4, 14.9, and 19.1±0.2 °2θ.
5 . The crystal form C of compound I according to claim 4 , characterized by an X ray diffraction pattern comprising three or more peaks at 2θ values selected from 7.4, 14.1, 14.9, 16.4, 19.1, 26.1, and 31.2±0.2 °2θ.
6 . A method of preparing a crystal form C of compound I according to claim 4 , comprising heating compound I in crystal form A to a temperature of at least about 250° C., or at least about 270° C., or about 280° C.
7 . A crystal form E of 4-(7-Hydroxy-2-isopropyl-4-oxo-4H-quinazolin-3-yl)benzonitrile (compound I) having structure
characterized by an X ray diffraction pattern comprising peaks at 2θ values of 12.7, 16.7, and 22.6±0.2 °2θ.
8 . The crystal form E of compound I according to claim 7 , characterized by an X ray diffraction pattern comprising three or more peaks at 2θ values selected from 12.7, 15.1, 16.7, 22.6, 27.1, 27.7, and 28.5±0.2 °2θ.
9 . A method of preparing a crystal form E of compound I according to claim 7 , comprising heating a hydrate form of compound I to temperatures greater than about 250° C. or about 260° C. to provide compound I as crystal form E.
10 . A crystalline hydrate of 4-(7-Hydroxy-2-isopropyl-4-oxo-4H-quinazolin-3-yl)-benzonitrile (compound I) having structure
11 . The crystalline hydrate of compound I according to claim 10 , characterized by an X ray diffraction pattern comprising peaks at 2θ values of 6.6, 14.4, and 18.3±0.2 °2θ.
12 . The crystalline hydrate of compound I according to claim 10 , characterized by an X ray diffraction pattern comprising three or more peaks at 2θ values selected from 6.6, 11.9, 14.4, 18.3, 23.9, 26.5, and 29.2±0.2 °2θ.
13 . A method of preparing the crystalline hydrate of compound I according to claim 10 , comprising equilibrating a slurry of compound I in a mixture of water and a water miscible solvent, to crystallize compound I as the crystalline hydrate.
14 . The method according to claim 13 , wherein the water miscible solvent is acetone.
15 . The method according to claim 13 , wherein the equilibration is carried out for about 12 hours, about 18 hours, or about 24 hours, or about 48 hours.
16 . A pharmaceutical formulation, comprising an effective amount of a crystal form of compound I selected from the group consisting of: crystal form A, crystal form C, crystal form D, crystal form E, and combinations thereof, and a pharmaceutically acceptable excipient.