Granted Patent B2

US 12,317,795 · App. 17/431,119 · Granted Jun 3, 2025

Inventors: Thi Ninh Thuan Nguyen (Deinze, BE); Godfrey Chongo (Saskatoon, CA); Jasper Devlamynck (Deinze, BE); Geoffrey Wagner (Zwijnaarde, BE)

Assignee: BASF Agricultural Solutions US LLC

A01H1/1245A01H5/06A01H6/202

Abstract

Claims (27)

1. A Brassica plant comprising <2% erucic acid in the seed oil, and comprising a CrS clubroot resistance locus in a chromosomal segment comprising the marker interval from marker M4 to marker M5,

wherein the marker M4 comprises a 5′ flanking sequence of SEQ ID NO: 7, a polymorphic base, and a 3′ flanking sequence of SEQ ID NO: 8, wherein the polymorphic base is A, and

wherein the marker M5 comprises a 5′ flanking sequence of SEQ ID NO: 9, a polymorphic base, and a 3′ flanking sequence of SEQ ID NO: 10, wherein the polymorphic base is T.

2. The Brassica plant according to claim 1 , which is a Brassica napus or a Brassica rapa plant.

3. The Brassica plant according to claim 2 , which is a Brassica napus WOSR plant or a Brassica napus SOSR plant.

4. The Brassica plant according to claim 1 , wherein said chromosomal segment is obtainable from reference seeds deposited at NCIMB under accession number NCIMB 43341.

5. The Brassica plant according to claim 1 , which is resistant to P. brassicae pathotypes P2, P3, P5, P6 or P8 or to isolate CR11.

6. The Brassica plant according to claim 1 , wherein said plant is heterozygous for said clubroot resistance locus.

7. The Brassica plant according to claim 1 , wherein said plant is homozygous for said clubroot resistance locus.

8. The Brassica plant according to claim 1 , said plant further comprising a gene conferring herbicide tolerance.

9. The Brassica plant according to claim 8 , wherein said gene conferring herbicide tolerance is a gene which confers resistance to glufosinate or to glufosinate ammonium or a gene conferring resistance to glyphosate.

10. Seeds of the Brassica plants according to claim 1 , wherein said seeds comprise a CrS clubroot resistance locus in a chromosomal segment comprising the marker interval from marker M4 to marker M5, and comprise <2% erucic acid in the seed oil,

wherein the marker M4 comprises a 5′ flanking sequence of SEQ ID NO: 7, a polymorphic base, and a 3′ flanking sequence of SEQ ID NO: 8, wherein the polymorphic base is A, and

wherein the marker M5 comprises a 5′ flanking sequence of SEQ ID NO: 9, a polymorphic base, and a 3′ flanking sequence of SEQ ID NO: 10, wherein the polymorphic base is T.

11. A method for producing a clubroot resistant Brassica plant, said method comprising

(a) identifying at least one Brassica plant comprising a CrS clubroot resistance locus in a chromosomal segment comprising the marker interval from M4 to M5, and

(b) selecting a plant comprising said CrS clubroot resistance locus,

wherein the marker M4 comprises a 5′ flanking sequence of SEQ ID NO: 7, a polymorphic base, and a 3′ flanking sequence of SEQ ID NO: 8, wherein the polymorphic base is A, and

wherein the marker M5 comprises a 5′ flanking sequence of SEQ ID NO: 9, a polymorphic base, and a 3′ flanking sequence of SEQ ID NO: 10, wherein the polymorphic base is T.

12. A method for producing a clubroot resistant Brassica plant, said method comprising

(a) crossing a first Brassica plant comprising a CrS clubroot resistance locus in a chromosomal segment comprising the marker interval from M4 to M5 with a second plant; and

(b) identifying a progeny plant comprising the marker interval from M4 to M5; or

comprising introducing the CrS clubroot resistance locus into a plant not comprising the CrS clubroot resistance locus using genome editing,

wherein the marker M4 comprises a 5′ flanking sequence of SEQ ID NO: 7, a polymorphic base, and a 3′ flanking sequence of SEQ ID NO: 8, wherein the polymorphic base is A, and

wherein the marker M5 comprises a 5′ flanking sequence of SEQ ID NO: 9, a polymorphic base, and a 3′ flanking sequence of SEQ ID NO: 10, wherein the polymorphic base is T.

13. A method for the protection of a group of cultivated plants according to claim 8 in a field comprising applying a composition comprising one or more herbicidal active ingredients.

14. The method according to claim 13 , wherein the herbicide is glufosinate or glufosinate ammonium or glyphosate.

Assignments (5)

Priority Claims (1)

Continuity (1)

References Cited (60)