PEPTIDES FOR PREVENTING BIOFILM FORMATION
The present invention relates to the use of a peptide to prevent biofilm formation by microorganisms on a surface, wherein said peptide is (i) a peptide consisting of 6 to 37 consecutive amino acids from a peptide of sequence SEQ ID NO: 1, (ii) a peptide of sequence SEQ ID NO: 4, or (iii) a peptidomimetic of (i) or (ii).
1 . A method for preventing biofilm formation by microorganisms on a surface, comprising the use of a peptide, wherein said peptide is
(i) a peptide consisting of 6 to 37, in particular 10 to 37, consecutive amino acids from a peptide of sequence SEQ ID NO: 1,
(ii) a peptide of sequence SEQ ID NO: 4, or
(iii) a peptidomimetic of (i) or (ii).
2 . The method according to claim 1 , wherein said peptide is a peptide consisting of an amino acid sequence selected from the group consisting of SEQ ID NOS: 2 and 3.
3 . The method according to claim 1 , wherein said peptide does not inhibit planktonic growth of the biofilm-forming microorganisms.
4 . The method according to claim 3 , wherein said microorganisms are antibiotic tolerant or antibiotic resistant bacteria.
5 . The method according to claim 3 , wherein said microorganisms are selected from the group consisting of Staphylococcus epidermis, Pseudomonas aeruginosa and Cryptococcus neoformans.
6 . The method according to claim 1 , wherein said surface is an abiotic or biotic surface.
7 . An isolated peptide consisting of an amino acid sequence selected from the group consisting of sequences SEQ ID NO: 2 (RSCQQQIQQAQQLSSCQQYLKQ), SEQ ID NO: 1 (RSCQQQIQQAQQLSSCQQYLKQRVQSEEGEDQISQRE), SEQ ID NO: 3 (RVQSEEGEDQISQRE), SEQ ID NO: 4 (RAEAFQTAQALPGLCRI), SEQ ID NO: 5 (LSSCQQYLKQ), SEQ ID NO: 6 (RSCQQQIQQAQQ), SEQ ID NO: 7 (RSCQQQ), SEQ ID NO: 8 (IQQAQQLS) and SEQ ID NO: 9 (SCQQYLKQ), or a peptidomimetic thereof.
8 . The isolated peptide according to claim 7 , consisting of an amino acid sequence selected from the group consisting sequences SEQ ID NO: 2 (RSCQQQIQQAQQLSSCQQYLKQ), SEQ ID NO: 1 (RSCQQQIQQAQQLSSCQQYLKQRVQSEEGEDQISQRE), SEQ ID NO: 3 (RVQSEEGEDQISQRE) and SEQ ID NO: 4 (RAEAFQTAQALPGLCRI), or a peptidomimetic thereof.
9 . A composition comprising the isolated peptide of claim 7 .
10 . The composition according to claim 9 wherein said peptide consists of an amino acid sequence which is SEQ ID NO: 2.
11 . The composition according to claim 9 or 10 , wherein the concentration of said peptide in the composition is 1-10 μM.
12 . An ex vivo method for preventing biofilm formation by microorganisms on a device comprising at least one surface, said method comprising the step of coating said at least one surface with a composition according to claim 9 .
13 . The method according to claim 12 , wherein said device is a medical or industrial device.
14 . A device comprising at least one surface coated with the peptide as defined in claim 1 .
15 . A method for preventing infections in a patient by the prevention of biofilm formation by microorganisms, comprising administering to said patient a peptide as defined in claim 7 .
16 . The method according to claim 15 , wherein said infection is endocarditis, osteomyelitis, chronic sinusitis, urethritis or periodontitis.