IP Library › Granted Patent US 12,214,027
Granted Patent B2
US 12,214,027 · App. 17/441,768 · Granted Feb 4, 2025

Stabilisation of live mollicutes bacteria in a liquid composition

Inventors: Martin Piest (Afferden, NL); Edwin Kets (Heilig Landstichting, NL); Paul Vermeij (St. Anthonis, NL); Marian Anna Catharina Jozefina Heijnen (Arcen, NL); Nöel Dautzenberg (Nijmegen, NL)
Assignee: INTERVET INC.
A61K39/0241A61K2039/52A61K2039/552
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12,214,027
App. No.
17/441,768
Granted
Feb 4, 2025
Kind
B2
Abstract

The present invention relates to a liquid composition of live Mollicutes bacteria and a stabiliser, whereby the stabiliser is a natural deep-eutectic solvent (NADES). In this liquid composition the live Mollicutes are stabilised without need for freezing or freeze-drying. This allows various advantageous uses in diagnostics and medicine, specifically as a liquid vaccine for use against infection or disease caused by Mollicutes bacteria, for human- or non-human animals.

Claims (16)

1. A liquid composition for protection against infection or disease caused by Mollicutes bacteria, comprising live Mollicutes bacteria and a stabiliser, wherein the stabiliser is a natural deep-eutectic solvent (NADES), wherein the composition has a water activity of 0.85 or less, and a water content of 50% w/w or less.

2. The liquid composition of claim 1 , wherein the NADES comprises an organic salt and a polyol.

3. The liquid composition of claim 1 , wherein the NADES comprises two or more compounds that are all polyols.

4. The liquid composition of claim 2 , wherein the polyol is a sugar or a sugar-alcohol.

5. The liquid composition of claim 4 , wherein the sugar is selected from sucrose and trehalose, or in that the sugar-alcohol is one or more selected from: erythritol, xylitol, and sorbitol.

6. The liquid composition of claim 2 , wherein the organic salt is one or more selected from salts of: betaine, proline, choline, methionine, and arginine.

7. The liquid composition of claim 1 , wherein the Mollicutes bacteria are attenuated in respect of their pathogenic properties.

8. A method for manufacturing the liquid composition of claim 1 , comprising the steps of;

a. providing the NADES, and

b. admixing the NADES with live Mollicutes bacteria, wherein the admixture has a water activity of 0.85 or less, and a water content of 50% w/w or less.

9. A liquid composition obtainable by the method of claim 8 .

10. A kit of parts comprising at least two containers: one container comprising the liquid composition of claim 1 , and one container comprising a liquid diluent.

11. A method of vaccinating a human or non-human animal target against infection or disease caused by Mollicutes bacteria, comprising administering to said target the liquid composition of claim 1 .

12. A method of vaccinating a human or non-human animal target against infection or disease caused by Mollicutes bacteria, comprising mixing from the kit of parts of claim 10 , the contents of the container comprising the liquid composition with the contents of the container comprising a liquid diluent to form a mixture and then administering the mixture to the human or non-human animal target.

13. A vaccine for protection against infection or disease caused by Mollicutes bacteria, comprising the liquid composition of claim 9 .

14. A method of vaccinating a human or non-human animal target against infection or disease caused by Mollicutes bacteria, comprising administering to the target the liquid vaccine of claim 13 .

Assignments (2)
CHANGE OF ADDRESS Recorded Sep 26, 2023
From: INTERVET INC.
To: INTERVET INC.
Reel/Frame 065028/0818 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 22, 2021
From: PIEST, MARTIN; KETS, EDWIN; VERMEIJ, PAUL; HEIJNEN, MARIAN ANNA CATHARINA JOZEFINA; DAUTZENBERG, NOEL
To: INTERVET INC.
Reel/Frame 057558/0147 →
Priority Claims (1)
EP 19166362 · Mar 29, 2019 · regional
Continuity (1)
Related Publication 20220175902A1 · Jun 9, 2022
References Cited (26)
US 4226940A · Storrs · 1980 [cited by applicant]
US 20140271710A1 · Buchanan et al. · 2014 [cited by applicant]
US 20150246114A1 · Qiao et al. · 2015 [cited by applicant]
CN 105992593A · 2016 [cited by applicant]
CN 105992593B · 2020 [cited by applicant]
JP 2014520807A · 2014 [cited by applicant]
JP 2017505807A · 2017 [cited by applicant]
WO WO2010037514A2 · 2010 [cited by applicant]
WO WO2010037514A3 · 2010 [cited by applicant]
WO WO2013006842A2 · 2013 [cited by applicant]
WO WO2013006842A3 · 2013 [cited by applicant]
WO 2015121463A2 · 2015 [cited by applicant]
WO 2016108083A1 · 2016 [cited by applicant]
WO 2017222377A1 · 2017 [cited by applicant]
WO WO2018211454A2 · 2018 [cited by applicant]
WO WO2018211454A3 · 2018 [cited by applicant]
Faggian, Marta, Natural Deep Eutectic Solvents (NADES) as a Tool for Bioavailability Improvement: Pharmacokinetics of Rutin Dissolved in Proline/Glycine after Oral Administration in Rats: Possible Application in Nutrace… [cited by applicant]
Gupta, R.S., et al., Phylogenetic framework for the phylum Tenericutes based on genome sequence data: proposal for the creation of a new order Mycoplasmoidales ord. nov., containing . . . , Antonie van Leeuwenhoek, 2018… [cited by applicant]
Gutiérrez, M.C., et al., Bacteria Incorporation in Deep-eutectic Solvents through Freeze-Drying, Wiley Interscience, Angew. Chem. Int. Ed., 2010, pp. 2158-2162, 49. [cited by applicant]
Paiva, Alexandre, Natural Deep Eutectic Solvents—Solvents for the 21st Century, ACS Sustainable Chemistry & Engineering, 2014, 1063-1071, 2(5). [cited by applicant]
Qiao, Y. et al., Effects of natural deep eutectic solvents on lactic acid bacteria viability during cryopreservation, Applied Microbiology and Biotechnology, 2018, pp. 1-11, https://doi.org/10.1007/s00253-018-8996-3. [cited by applicant]
Dai et al., 2015, “Tailoring properties of natural deep eutectic solvents with water to facilitate their applications,” Food Chem., 187:14-19. [cited by applicant]
Li et al., 2018, “Protective Effect of Natural Deep Eutectic Solvent on Green Fluorescent Protein,” Modern Food Science and Technology, 34(10):44-48, in Chinese with English abstract. [cited by applicant]
Meng et al., 2015, “Research Progress in Isolation and Identification Methods of Mycoplasma in Animals,” Shanghai Animal Husbandry and Veterinary Newsletter, 2:27-29, in Chinese with machine English translation. [cited by applicant]
Qin et al., 2002, “Current Status and Problems of Molecular Classification in the Phytoplasmas,” Scientia Silvae Sinicae, 38(6):125-136, in Chinese with English abstract. [cited by applicant]
Radosevic et al., 2018, “Antimicrobial, cytotoxic and antioxidative evaluation of natural deep eutectic solvents,” Environ. Sci. Pollut. Res. Int., 25(14):14188-14196. [cited by applicant]