X-aptamers for the use in detection of SNAP25
Described are methods for utilizing X-aptamers for detecting human SNAP25 and fragments thereof, and compositions comprising the X-aptamers.
1. A synthetic oligonucleotide sequence selected from the group consisting of SEQ ID NOs:1-96.
2. The oligonucleotide sequence according to claim 1 , wherein a modified nucleotide (n) in the synthetic oligonucleotide sequence is a deoxyuridine.
3. The oligonucleotide sequence according to claim 1 , wherein a modified nucleotide (n) in the synthetic oligonucleotide sequence is a deoxyribonucleotide triphosphate.
4. The oligonucleotide sequence according to claim 1 , wherein the synthetic oligonucleotide is bound to an additional molecule.
5. The oligonucleotide sequence according to claim 4 , wherein the additional molecule is selected from the group consisting of SNAP25 or a fragment thereof, a nucleic acid sequence, an amino acid sequence, a protein, an enzyme, a quantum dot, a fluorescent molecule, a dye, a chelator, a magnetic particle, and a linker capable of linking to another molecule.
6. The oligonucleotide sequence according to claim 5 , wherein the fluorescent molecule is a fluorescent protein.
7. A composition comprising a synthetic oligonucleotide sequence selected from the group consisting of SEQ ID NOs:1-96.
8. A kit comprising a synthetic oligonucleotide sequence selected from the group consisting of SEQ ID NOs:1-96.
9. A method of isolating SNAP25, or a fragment thereof, the method comprising:
(a) administering a composition comprising SNAP25, or a fragment thereof, to a composition comprising one or more synthetic oligonucleotide sequences selected from the group consisting of SEQ ID NOs:1-96, wherein the synthetic oligonucleotide sequences are immobilized;
(b) washing impurities from the SNAP25 or the fragment thereof bound to the immobilized synthetic oligonucleotide; and
(c) eluting the SNAP25 or the fragment thereof from the immobilized synthetic oligonucleotide sequences.
10. A method of binding SNAP25, or a fragment thereof, with one or more X-aptamers, the method comprising administering a composition comprising one or more synthetic X-aptamers selected from the group consisting of SEQ ID NOs:1-96 to a composition comprising the SNAP25 or the fragment thereof, and wherein the one or more synthetic X-aptamers bind to the SNAP25 or the fragment thereof.
11. The method according to claim 10 , wherein the composition comprising the SNAP25 or the fragment thereof is a composition in vivo.
12. The method according to claim 10 , wherein the composition comprising the SNAP25 or the fragment thereof is a composition in vitro.
13. The method according claim 10 , wherein the composition comprising the SNAP25 or the fragment thereof is a composition in situ.
14. The method according claim 10 , wherein the composition comprising the SNAP25 or the fragment thereof is a tissue sample.
15. The method according claim 10 , wherein the composition comprising the SNAP25 or the fragment thereof is a tissue culture.
16. The method according claim 10 , wherein the composition comprising the SNAP25 or the fragment thereof is a cell culture.
17. The method according claim 10 , wherein the composition comprising the SNAP25 or the fragment thereof is a gel.
18. The method according claim 10 , wherein the one or more X-aptamers are bound to an additional molecule.
19. The method according claim 18 , wherein the additional molecule is selected from the group consisting of a nucleic acid sequence, an amino acid sequence, a protein, an enzyme, a quantum dot, a fluorescent molecule, a fluorescent protein, a dye, a chelator, a magnetic particle, and a linker capable of linking to another molecule.