Solid substrates for promoting cell and tissue growth
This invention provides solid substrates for promoting cell or tissue growth or restored function, which solid substrate is characterized by a specific fluid uptake capacity value of at least 75%, which specific fluid uptake capacity value is determined by establishing a spontaneous fluid uptake value divided by a total fluid uptake value. This invention also provides solid substrates for promoting cell or tissue growth or restored function, which solid substrate is characterized by having a contact angle value of less than 60 degrees, when in contact with a fluid. This invention also provides solid substrates for promoting cell or tissue growth or restored function, which said substrate is characterized by a substantial surface roughness (Ra) as measured by scanning electron microscopy or atomic force microscopy. The invention also provides for processes for selection of an optimized coral-based solid substrate for promoting cell or tissue growth or restored function and applications of the same.
1. A process of promoting cartilage and/or bone repair or repair of an osteochondral defect, the process comprising implanting, in a subject, a solid substrate within a site in need of cartilage and/or bone repair, or in need of repair of an osteochondral defect,
wherein the solid substrate is a marine organism skeletal derivative-based solid material that comprises a coral or a coral-based derivative,
and wherein the specific fluid uptake capacity of the solid substrate is at least 75% when the specific fluid uptake capacity is determined by establishing the spontaneous fluid uptake value in water, establishing the total fluid uptake value in water under pressure, and dividing the spontaneous fluid uptake value by the total fluid uptake value.
2. The process of claim 1 , wherein the marine organism skeletal derivative-based solid material comprises the coral-based derivative.
3. The process of claim 2 , wherein the coral-based derivative comprises aragonite, calcite, mixtures thereof, or other polymorphs of the same.
4. The process of claim 2 , wherein the coral-based derivative comprises aragonite, calcite, hydroxyapatite, or a combination thereof.
5. The process of claim 1 , wherein the marine organism skeletal derivative-based solid material comprises a bone filler or bone substitute material.
6. The process of claim 1 , wherein the marine organism skeletal derivative-based solid material comprises a biocompatible polymer.
7. The process of claim 6 , wherein the biocompatible polymer is incorporated within voids or pores in the solid substrate or wherein the biocompatible polymer is attached to an outer surface of the solid substrate.
8. The process of claim 6 , wherein the biocompatible polymer comprises a natural polymer comprising a glycosaminoglycan, collagen, fibrin, elastin, silk, chitosan, alginate, and any combination thereof.
9. The process of claim 8 , wherein the biocompatible polymer comprises a glycosaminoglycan and the glycosaminoglycan is hyaluronic acid, sodium hyaluronate, cross linked hyaluronic acid, or a combination thereof.
10. The process of claim 1 , wherein the marine organism skeletal derivative-based solid material further comprises a cytokine, a growth factor, a therapeutic compound, an osteoinductive agent, a bioactive glass, a bone filler, a bone cement, a drug, or any combination thereof, wherein the therapeutic compound or drug comprises an anti-inflammatory compound, an anti-infective compound, a pro-angiogenic factor or a combination thereof.
11. The process of claim 1 , wherein the marine organism skeletal derivative-based solid material approximates the form of a cylinder, cone, tac, pin, screw, rectangular bar, plate, disc, pyramid, granule, powder, coral sand, condyle, rib, pelvis, vertebra, bone, cartilaginous tissue, ball or cube.
12. The process of claim 1 , wherein the marine organism skeletal derivative-based solid material approximates a shape that accommodates a site of desired tissue growth or repair.
13. The process of claim 1 , wherein the marine organism skeletal derivative-based solid material comprises a hollow or hollows along a Cartesian coordinate axis of the solid material.
14. The process of claim 1 , wherein the process further comprises the step of contacting the marine organism skeletal derivative-based solid material with cells or tissue.
15. The process of claim 14 , wherein the process comprises contacting the marine organism skeletal derivative-based solid material with cells and the cells comprise stem or progenitor cells or a combination thereof.
16. The process of claim 1 , wherein the subject is a human subject.
17. The process of claim 1 , wherein the subject is a veterinary subject.
18. The process of claim 1 , wherein the subject is afflicted with a defect or disorder or disease of the cartilage or bone or a combination thereof.
19. The process of claim 18 , wherein the cartilage defect or disorder or disease comprises a full or partial thickness articular cartilage defect; osteochondral defect; osteoarthritis, a joint defect or a defect resulting from trauma, sports, or repetitive stress.
20. The process of claim 18 , wherein the defect or disorder or disease of the bone comprises a fracture, bone defect, bone edema, osteoporosis, bone tumor, bone cyst, or a defect resulting from trauma, sports, or repetitive stress.
21. The process of claim 1 , wherein the marine organism skeletal derivative-based solid material contains ground particles derived from the coral-based derivative, suspended in a biocompatible matrix or further comprises a bone filler or bone substitute material.
22. The process of claim 21 , wherein the biocompatible matrix is a hydrogel.