IP Library Granted Patent US 12,215,134
Granted Patent B2
US 12,215,134 · App. 17/480,473 · Granted Feb 4, 2025

Engineered immune cells with dominant signals

Inventor: Alexander Kamb (Agoura Hills, CA)
Assignee: A2 BIOTHERAPEUTICS, INC.
C07K14/7051A61K35/17A61K39/4611A61K39/4631A61K39/4632A61K39/464404A61K39/464486A61K39/464488C07K14/4702C12N5/0637C12N15/85C07K2317/53C07K2317/622C07K2319/03C07K2319/715C12N2510/00
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Quick Facts
Patent No.
US 12,215,134
App. No.
17/480,473
Granted
Feb 4, 2025
Kind
B2
Abstract

The present disclosure provides engineered immune cells and methods for their creation and use. The immune cells comprise activating and blocking receptors, in which the blocking receptor provides a signal that dominates a signal from the activating receptor.

Claims (13)

1. A method for reducing tumor size or increasing survival time of a patient with a cancer, the method comprising:

providing an engineered immune cell to the patient, wherein the engineered immune cell comprises an activating receptor and a blocking receptor, each expressed on a surface of the engineered immune cell, said blocking receptor comprising a hinge that comprises a peptide, wherein said peptide comprises a continuous stretch of at least 24 amino acids of SEQ ID NO: 84 derived from a leukocyte immunoglobulin-like receptor subfamily B member 1 (LILRB1), wherein:

when the engineered immune cell encounters a tumor cell, the activating receptor binds to a first ligand on the tumor cell and the activating receptor triggers an activating signal in the engineered immune cell that promotes a cytotoxic response by the engineered immune cell that results in a cytotoxic effect on the tumor cell; and

when the engineered immune cell encounters a normal cell, the activating receptor binds to the first ligand on the normal cell and the blocking receptor binds to a second ligand on the normal cell, wherein the activating receptor triggers the activating signal in the engineered immune cell and the blocking receptor triggers an inhibitory signal in the engineered immune cell that is stronger than the activating signal such that the inhibitory signal dominates and blocks the activating signal from the activating receptor, thereby preventing a localized cytotoxic response by the engineered immune cell.

2. The method of claim 1 , wherein binding of the blocking receptor to the second ligand causes the engineered immune cell to exhibit reduced surface expression of the activating receptor.

3. The method of claim 2 , wherein the reduced surface expression is reversible.

4. The method of claim 1 , wherein the immune cell expresses different concentrations of the activating and blocking receptors based on a quantity of the first ligand and a quantity of the second ligand expressed in the normal cell of the patient.

5. The method of claim 4 , wherein a ratio of the concentration of the blocking receptors expressed to the activating receptors expressed is less than or equal to 1.

6. The method of claim 1 , wherein when the immune cell encounters the tumor cell, a first plurality of the activating receptors diffuses into a first region on the surface of the immune cell proximal to the tumor cell and forms a first micro-cluster that promotes the cytotoxic response by the immune cell that results in the cytotoxic effect on the tumor cell.

7. The method of claim 6 , wherein when the immune cell simultaneously encounters the normal cell, a plurality of the activating and blocking receptors diffuses into a second region on the surface of the immune cell proximal to the normal cell and forms a second micro-cluster causing the inhibitory signal from the blocking receptors to dominate the activating signal from the activating receptors in the second micro-cluster preventing the localized cytotoxic response by the engineered immune cell on the normal cell.

8. The method of claim 7 , wherein binding of the blocking receptors in the second micro-cluster to the second ligand prevents breakup of the second micro-cluster.

9. The method of claim 1 , wherein the blocking receptor does not bind to the second ligand until the activating receptor binds to the first ligand.

10. The method of claim 1 , wherein cross-talk between the activating receptor and the blocking receptor affects an activation threshold for the localized cytotoxic response.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 20, 2021
From: KAMB, ALEXANDER
To: A2 BIOTHERAPEUTICS, INC.
Reel/Frame 058431/0238 →
Continuity (9)
Provisional Application 63081250 · Sep 21, 2020
Provisional Application 63081229 · Sep 21, 2020
Provisional Application 63081242 · Sep 21, 2020
Provisional Application 63081258 · Sep 21, 2020
Provisional Application 63081231 · Sep 21, 2020
Provisional Application 63081256 · Sep 21, 2020
Provisional Application 63081237 · Sep 21, 2020
Provisional Application 63081248 · Sep 21, 2020
Related Publication 20220089675A1 · Mar 24, 2022
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