IP Library Patent Application 17483274
Patent Application
App. No. 17/483,274

AFFINITY-OLIGONUCLEOTIDE CONJUGATES AND USES THEREOF

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Quick Facts
Patent No.
US None
App. No.
17/483,274
Abstract

Provided herein are methods and compositions for single cell characterization using affinity-oligonucleotide conjugates.

Claims (10)

1 - 124 . (canceled)

125 . A kit for making conjugates, comprising:

(a) a first container comprising a first oligonucleotide comprising a first antigen identification (AID) sequence and a first affinity molecular barcode (AMB) sequence, wherein the first AID sequence and the first AMB sequence are known sequences;

(b) a second container comprising a second oligonucleotide comprising a second antigen identification (AID) sequence and a second affinity molecular barcode (AMB) sequence, wherein the second AID sequence is a known sequence and is different from the first AID sequence, and wherein the second AMB sequence is a known sequence and is different from the first AMB sequence; and

(c) one or more additional containers comprising reagents capable of conjugating the first oligonucleotide to a first affinity molecule and/or comprising reagents capable of conjugating the second oligonucleotide to a second affinity molecule;

wherein the first affinity molecule is configured to bind to a first extracellular protein of a cell, and the second affinity molecule is configured to bind to a second extracellular protein of a cell, wherein the first extracellular protein is different from the second extracellular protein; and

(d) a set of instructions describing how to conjugate the first oligonucleotide to the first affinity molecule and the second oligonucleotide to the second affinity molecule.

126 . The kit of claim 125 , further comprising:

(e) a further container comprising a plurality of vessel barcoded polynucleotides, wherein each vessel barcoded polynucleotide of the plurality of vessel barcoded polynucleotides comprises a unique vessel barcoded sequence; and

(f) reagents and a set of instructions to attach a vessel barcoded polynucleotide of the plurality of vessel barcoded polynucleotides to the first or second oligonucleotide when conjugated to the first or second affinity molecule.

Assignments (2)
ASSIGNEE CHANGE OF ADDRESS Recorded Apr 19, 2023
From: ABVITRO LLC
To: ABVITRO LLC
Reel/Frame 063396/0161 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 11, 2023
From: VIGNEAULT, FRANCOIS; BRIGGS, ADRIAN WRANGHAM; GOLDFLESS, STEPHEN J.; BELMONT, BRIAN J.
To: ABVITRO LLC
Reel/Frame 063299/0495 →