Multivalent feline vaccine
The present invention provides new multivalent vaccines for felines. The present invention also provides methods of making and using the multivalent vaccines alone or in combinations with other protective agents.
1. A vaccine to aid in the prevention of disease due to feline calicivirus (FCV) comprising an alphavirus RNA replicon particle that encodes one or more FCV capsid proteins and a modified live feline pathogen selected from the group consisting of a modified live feline viral rhinotracheitis virus (FVR), a modified live feline panleukopenia virus (FPLV), a modified live Chlamydophila felis , and any combination thereof, and a pharmaceutically acceptable carrier.
2. The vaccine of claim 1 , wherein the one or more FCV capsid proteins is selected from the group consisting of a FCV F9-Like capsid protein, a virulent systemic FCV (VS-FCV) capsid protein, or both a FCV F9-Like capsid protein and a virulent systemic FCV (VS-FCV) capsid protein.
3. The vaccine of claim 2 , wherein the alphavirus RNA replicon particle is a Venezuelan Equine Encephalitis (VEE) alphavirus RNA replicon particle.
4. A method of immunizing a feline against a pathogenic FCV comprising administering to the feline an immunologically effective amount of the vaccine of claim 3 .
5. The vaccine of claim 3 , wherein the VEE alphavirus RNA replicon particle also encodes an antigen selected from the group consisting of a feline leukemia virus (FeLV) glycoprotein gp85, a FeLV glycoprotein gp70, a FeLV glycoprotein gp45, a rabies virus G protein, or any combination thereof.
6. The vaccine of claim 5 , wherein the antigen is selected from the group consisting of an FeLV gp85 that comprises an amino acid sequence comprising at least 95% identity with the amino acid sequence of SEQ ID NO: 6, an FeLV gp70 that comprises an amino acid sequence comprising 95% identity or more with the amino acid sequence of SEQ ID NO: 8, and the combination thereof.
7. The vaccine of claim 5 , wherein the antigen is a rabies virus G protein that comprises an amino acid sequence comprising at least 95% identity with the amino acid sequence of SEQ ID NO: 10.
8. The vaccine of claim 3 , that further comprises an additional VEE alphavirus RNA replicon particle that encodes an antigen selected from the group consisting of a feline leukemia virus (FeLV) glycoprotein gp85, a FeLV glycoprotein gp70, a FeLV glycoprotein gp45, a rabies virus G protein, or any combination thereof.
9. The vaccine of claim 8 , wherein the antigen is selected from the group consisting of an FeLV gp85 that comprises an amino acid sequence comprising at least 95% identity with the amino acid sequence of SEQ ID NO: 6, an FeLV gp70 that comprises an amino acid sequence comprising 95% identity or more with the amino acid sequence of SEQ ID NO: 8, and the combination thereof.
10. The vaccine of claim 8 , wherein the antigen is a rabies virus G protein that comprises an amino acid sequence comprising at least 95% identity with the amino acid sequence of SEQ ID NO: 10.
11. The vaccine of claim 3 , wherein the FCV capsid protein is a VS-FCV capsid protein that comprises an amino acid sequence comprising at least 95% identity with the amino acid sequence of SEQ ID NO: 2.
12. The vaccine of claim 3 , wherein the FCV capsid protein is a FCV F9-Like capsid protein that comprises an amino acid sequence comprising at least 95% identity with the amino acid sequence of SEQ ID NO: 4.
13. A vaccine to aid in the prevention of disease due to FCV comprising a Venezuelan Equine Encephalitis (VEE) alphavirus RNA replicon particle that encodes a virulent systemic feline calicivirus (VS-FCV) capsid protein, a modified live feline viral rhinotracheitis virus (FVR), a modified live feline panleukopenia virus (FPLV), a modified live Chlamydophila felis and a pharmaceutically acceptable carrier; wherein the VS-FCV capsid protein comprises an amino acid sequence comprising at least 95% identity with the amino acid sequence of SEQ ID NO: 2.
14. A method of immunizing a feline against a pathogenic FCV comprising administering to the feline an immunologically effective amount of the vaccine of claim 13 .
15. The vaccine of claim 13 , wherein the VEE alphavirus RNA replicon particle further encodes a feline calicivirus F9-Like capsid protein; wherein the FCV F9-Like capsid protein comprises an amino acid sequence comprising at least 95% identity with the amino acid sequence of SEQ ID NO: 4.
16. The vaccine of claim 15 , further comprising a Venezuelan Equine Encephalitis (VEE) alphavirus RNA replicon particle that encodes a feline leukemia virus (FeLV) glycoprotein gp85, a FeLV glycoprotein gp70, or both; wherein the FeLV gp85 comprises an amino acid sequence comprising at least 95% identity with the amino acid sequence of SEQ ID NO: 6 and the FeLV gp70 comprises an amino acid sequence comprising 95% identity or more with the amino acid sequence of SEQ ID NO: 8.
17. A method of immunizing a feline against a pathogenic FCV comprising administering to the feline an immunologically effective amount of the vaccine of claim 16 .
18. A method of immunizing a feline against a pathogenic FCV comprising administering to the feline an immunologically effective amount of the vaccine of claim 15 .
19. The vaccine of claim 13 , further comprising a VEE alphavirus RNA replicon particle that encodes a feline calicivirus F9-Like capsid protein; wherein the FCV F9-Like capsid protein comprises an amino acid sequence comprising at least 95% identity with the amino acid sequence of SEQ ID NO: 4.
20. A method of immunizing a feline against a pathogenic FCV comprising administering to the feline an immunologically effective amount of the vaccine of claim 19 .