Primer set for detecting SARS-CoV-2, method for testing SARS-CoV-2, and reagent and kit of testing SARS-CoV-2

1. A primer set for detecting SARS-CoV-2, the primer set comprising a plurality of LAMP primers targeting one or more open reading frame (ORF) regions in a SARS-CoV-2 genome,

wherein the primer set comprises one or more primer sets selected from the group consisting of the following (1) to (4):

(1) a primer set comprising four primers comprising the nucleotide sequences of SEQ ID NOs:13-16 or the nucleotide sequences complementary thereto, respectively;

(2) a primer set comprising four primers comprising the nucleotide sequences of SEQ ID NOs:19-22 or the nucleotide sequences complementary thereto, respectively;

(3) a primer set comprising four primers comprising the nucleotide sequences of SEQ ID NOs:25-28 or the nucleotide sequences complementary thereto, respectively; and

(4) a primer set comprising four primers comprising the nucleotide sequences of SEQ ID NOs:43-46 or the nucleotide sequences complementary thereto, respectively.

2. The primer set according to claim 1 , wherein the primer set comprises the primer set of either (1) or (2) or the primer sets of both (1) and (2).

3. The primer set according to claim 1 , wherein the primer set comprises the primer set of (1).

4. A method for testing SARS-CoV-2, the method comprising detecting SARS-CoV-2 in a specimen obtained from a subject by using the primer set according to claim 1 in an RT-LAMP method.

5. The method according to claim 4 , wherein the specimen is nasal swab, pharyngeal swab, saliva, or sputum.

6. The method according to claim 4 , wherein SARS-CoV-2 is detected after heating the specimen.

7. A reagent of testing SARS-CoV-2, comprising the primer set according to claim 1 .

8. A kit of testing SARS-CoV-2, comprising the following (1) to (3):

(1) the primer set according to claim 1 ;

(2) a strand-displacement DNA polymerase; and

(3) a reverse transcriptase.

9. A primer set for detecting SARS-CoV-2, the primer set comprising a plurality of LAMP primers targeting one or more open reading frame (ORF) regions in a SARS-CoV-2 genome,

wherein the primer set comprises one or more primer sets selected from the group consisting of the following (1) to (4):

(1) a primer set comprising six primers comprising the nucleotide sequences of SEQ ID NOs:13-18 or the nucleotide sequences complementary thereto, respectively;

(2) a primer set comprising six primers comprising the nucleotide sequences of SEQ ID NOs:19-24 or the nucleotide sequences complementary thereto, respectively;

(3) a primer set comprising six primers comprising the nucleotide sequences of SEQ ID NOs:25-30 or the nucleotide sequences complementary thereto, respectively; and

(4) a primer set comprising six primers comprising the nucleotide sequences of SEQ ID NOs:43-48 or the nucleotide sequences complementary thereto, respectively.

10. The primer set according to claim 9 , wherein the primer set comprises the primer set of either (1) or (2) or the primer sets of both (1) and (2).

11. The primer set according to claim 9 , wherein the primer set comprises the primer set of (1).

12. A method for testing SARS-CoV-2, the method comprising detecting SARS-CoV-2 in a specimen obtained from a subject by using the primer set according to claim 9 in an RT-LAMP method.

13. The method according to claim 12 , wherein the specimen is nasal swab, pharyngeal swab, saliva, or sputum.

14. The method according to claim 12 , wherein SARS-CoV-2 is detected after heating the specimen.

15. A reagent of testing SARS-CoV-2, comprising the primer set according to claim 9 .

16. A kit of testing SARS-CoV-2, comprising the following (1) to (3):

(1) the primer set according to claim 9 ;

(2) a strand-displacement DNA polymerase; and

(3) a reverse transcriptase.