OXYGEN-INSENSITIVE ELECTROCHEMICAL BIOSENSOR AND METHODS OF USE THEREOF
The present disclosure provides a reagent for detecting an analyte, comprising: an enzyme comprising glucose oxidoreductase; at least one electroactive molecule selected from resazurin, resazurin salt, resorufin or resorufin salt; and at least one metal coordination complex.
1 . A reagent for detecting an analyte, comprising:
an enzyme comprising glucose oxidoreductase;
at least one electroactive molecule selected from resazurin, resazurin salt, resorufin or resorufin salt; and
at least one metal coordination complex.
2 . The reagent according to claim 1 , wherein the glucose oxidoreductase is glucose dehydrogenase.
3 . The reagent according to claim 1 , wherein the at least one metal coordination complex comprises a ruthenium complex or an osmium complex.
4 . The reagent according to claim 1 , wherein the at least one electroactive molecule is provided in concentration of between 0.01 mM and about 20 mM.
5 . The reagent according to claim 1 , wherein the at least one electroactive molecule comprises resazurin or resazurin salt is provided in concentration of between 1 mM and about 3 mM.
6 . The reagent according to claim 1 , wherein the at least one electroactive molecule comprises resorufin or resorufin salt is provided in concentration of between 1 mM and about 4 mM.
7 . The reagent according to claim 1 , further comprising a coenzyme comprising flavin adenine dinucleotide or nicotinamide adenine dinucleotide.
8 . A test strip for detecting an analyte, comprising:
a substrate with a proximal region and a distal region;
a conductive pattern formed on the substrate including at least one electrode disposed on the substrate at the proximal region of the substrate, electrical strip contacts disposed on the substrate at a conductive region at the distal region of the substrate, and conductive traces electrically connecting the at least one electrode to at least some of the electrical strip contacts; and
a reagent layer contacting at least a portion of at least one electrode, the reagent layer comprising:
a glucose oxidoreductase;
a mediator formulation comprising at least one electroactive molecule independently selected from resazurin, resazurin salt, resorufin or resorufin salt; and
at least one metal coordination complex.
9 . The test strip according to claim 8 , wherein the glucose oxidoreductase is glucose dehydrogenase.
10 . The test strip according to claim 8 , wherein the at least one metal coordination complex comprises a ruthenium complex or an osmium complex.
11 . The test strip according to claim 8 , wherein the at least one electroactive molecule is provided in concentration of between 0.01 mM and about 20 mM.
12 . The test strip according to claim 8 , wherein the at least one electroactive molecule comprises resazurin or resazurin salt is provided in concentration of between 1 mM and about 3 mM.
13 . The test strip according to claim 8 , wherein the at least one electroactive molecule comprises resorufin or resorufin salt is provided in concentration of between 1 mM and about 4 mM.
14 . The test strip according to claim 8 , further comprising a coenzyme comprising flavin adenine dinucleotide or nicotinamide adenine dinucleotide.
15 . A system for measuring a body fluid constituent comprising:
a test strip for detecting an analyte comprising:
a substrate with a proximal region and a distal region;
a conductive pattern formed on the substrate including at least one electrode disposed on the substrate at the proximal region of the substrate, electrical strip contacts disposed on the substrate at a conductive region at the distal region of the substrate, and conductive traces electrically connecting the at least one electrode to at least some of the electrical strip contacts; and
a reagent layer contacting at least a portion of at least one electrode, the reagent layer comprising:
a glucose oxidoreductase;
a mediator formulation comprising at least one electroactive molecule independently selected from resazurin, resazurin salt, resorufin or resorufin salt; and
at least one metal coordination complex; and
a diagnostic meter configured to receive the test strip and to electrically connect to the electrical strip contacts.
16 . The system according to claim 15 , wherein the glucose oxidoreductase is glucose dehydrogenase.
17 . The system according to claim 15 , wherein the at least one metal coordination complex comprises a ruthenium complex or an osmium complex.
18 . The system according to claim 15 , wherein the at least one electroactive molecule is provided in concentration of between 0.01 mM and about 20 mM.
19 . The system according to claim 15 , wherein the at least one electroactive molecule comprises resazurin or resazurin salt is provided in concentration of between 1 mM and about 3 mM.
20 . The system according to claim 15 , wherein the at least one electroactive molecule comprises resorufin or resorufin salt is provided in concentration of between 1 mM and about 4 mM.