IP Library › Granted Patent US 12,365,870
Granted Patent B2
US 12,365,870 · App. 17/504,067 · Granted Jul 22, 2025

Storage stabilizer for extracellular vesicle and storage stabilization method for extracellular vesicle

Inventors: Masayuki Yamane (Amagasaki, JP); Naoko Imawaka (Amagasaki, JP); Yuji Nakagawa (Osaka, JP); Kodai Sasamoto (Amagasaki, JP)
Assignee: FUJIFILM Corporation
C12N5/00C08F126/10
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Quick Facts
Patent No.
US 12,365,870
App. No.
17/504,067
Granted
Jul 22, 2025
Kind
B2
Abstract

An object of the present invention is to provide a means capable of stably storing an extracellular vesicle. The present invention relates to a storage stabilizer for an extracellular vesicle, which contains a copolymer containing a monomer unit derived from vinylpyrrolidone and a monomer unit derived from vinyl acetate, or a polymer having a monomer unit derived from vinylpyrrolidone such as polyvinylpyrrolidone and relates to a storage stabilization method for an extracellular vesicle, which includes cryopreserving an extracellular vesicle in the presence of the polymer.

Claims (12)

1. A storage stabilization method for an extracellular vesicle, comprising cryopreserving an extracellular vesicle in a presence of a polymer having a monomer unit derived from vinylpyrrolidone,

wherein the polymer is a copolymer containing a monomer unit derived from vinylpyrrolidone and a monomer unit derived from vinyl acetate, or polyvinylpyrrolidone having a weight-average molecular weight of 1,500 to 15,000;

the cryopreserving of an extracellular vesicle is cryopreserving a storage solution comprising the polymer, the extracellular vesicle, and an aqueous solution; and

the concentration of the polymer in the storage solution is 0.001 to 1% (w/v).

2. The storage stabilization method for an extracellular vesicle according to claim 1 , wherein the polymer is a copolymer containing a monomer unit derived from vinylpyrrolidone and a monomer unit derived from vinyl acetate.

3. The storage stabilization method for an extracellular vesicle according to claim 2 , wherein a constitution ratio of the monomer unit derived from vinylpyrrolidone to the monomer unit derived from vinyl acetate in the copolymer is 1:0.1 to 1:3.

4. The storage stabilization method for an extracellular vesicle according to claim 2 , wherein a Fikentscher K value of the copolymer is 5 to 50.

5. The storage stabilization method for an extracellular vesicle according to claim 2 , wherein a weight-average molecular weight of the copolymer is 3,000 to 250,000.

6. The storage stabilization method for an extracellular vesicle according to claim 1 , wherein the polymer is polyvinylpyrrolidone.

7. The storage stabilization method for an extracellular vesicle according to claim 6 , wherein a Fikentscher K value of the polyvinylpyrrolidone is 9 to 20.

8. The storage stabilization method for an extracellular vesicle according to claim 1 , wherein the extracellular vesicle is an isolated extracellular vesicle.

9. The storage stabilization method for an extracellular vesicle according to claim 1 , which further comprises isolating an extracellular vesicle from a biological specimen prior to cryopreserving the extracellular vesicle.

Assignments (3)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 6, 2026
From: FUJIFILM CORPORATION
To: FUJIFILM WAKO PURE CHEMICAL CORPORATION
Reel/Frame 073999/0664 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 30, 2022
From: FUJIFILM WAKO PURE CHEMICAL CORPORATION
To: FUJIFILM CORPORATION
Reel/Frame 060939/0900 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 19, 2021
From: YAMANE, MASAYUKI; IMAWAKA, NAOKO; NAKAGAWA, YUJI; SASAMOTO, KODAI
To: FUJIFILM WAKO PURE CHEMICAL CORPORATION
Reel/Frame 057836/0957 →
Priority Claims (1)
JP 2019-080005 · Apr 19, 2019 · national
Continuity (2)
Continuation PCTJP2020016738 · Apr 16, 2020
Related Publication 20220033765A1 · Feb 3, 2022
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