Methods And Compositions For Producing Hydrocarbons
Compositions and methods for producing aldehydes, alkanes, and alkenes are described herein. The aldehydes, alkanes, and alkenes can be used in biofuels.
1 .- 43 . (canceled)
44 . A recombinant microorganism for the production of a hydrocarbon, wherein the recombinant microorganism expresses:
a polynucleotide sequence encoding a decarbonylase;
a polynucleotide sequence encoding a ferredoxin; and
a polynucleotide sequence encoding a ferredoxin reductase.
45 . The recombinant microorganism of claim 44 , wherein the decarbonylase comprises:
an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, or 36; or
an amino acid sequence having at least 85% sequence identity to SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, or 36.
46 . The recombinant microorganism of claim 44 , wherein the ferredoxin comprises:
an amino acid sequence set forth in SEQ ID NO: 90, 92, or 94; or
an amino acid sequence having at least 85% sequence identity to SEQ ID NO: 90, 92, or 94.
47 . The recombinant microorganism of claim 44 , wherein the ferredoxin reductase comprises:
an amino acid sequence set forth in SEQ ID NO:88; or
an amino acid sequence having at least 85% sequence identity to SEQ ID NO:88.
48 . The recombinant microorganism of claim 44 , comprising a polynucleotide sequence encoding an acyl-ACP reductase (AAR).
49 . The recombinant microorganism of claim 48 , wherein the AAR comprises an amino acid sequence having at least 85% sequence identity to SEQ ID NO: 66, 68, 70, 72, 74, 76, 78, 80, or 82.
50 . The recombinant microorganism of claim 44 , comprising a polynucleotide sequence encoding a carboxylic acid reductase (CAR).
51 . The recombinant microorganism of claim 50 , wherein the CAR comprises an amino acid sequence having at least 85% sequence identity to SEQ ID NO:86.
52 . The recombinant microorganism of claim 50 , further comprising a polynucleotide sequence encoding a thioesterase.
53 . The recombinant microorganism of claim 52 , wherein the thioesterase is TesA without the leader sequence (TesA), TesB, FatA, FatA1, FatB, FatB2, or FatB3.
54 . The recombinant microorganism of claim 44 , wherein the recombinant microorganism is selected from the group consisting of a bacterium, yeast, fungus, and algae.
55 . The recombinant microorganism of claim 54 , wherein the recombinant microorganism is a bacterium.
56 . The recombinant microorganism of claim 55 , wherein the bacterium is E. coli.
57 . The recombinant microorganism of claim 55 , wherein the bacterium is a cyanobacterium.
58 . The recombinant microorganism of claim 44 , wherein the recombinant microorganism produces a hydrocarbon that is a C 3 to C 21 alkane or alkene.
59 . The recombinant microorganism of claim 58 , wherein the alkane or alkene is tridecane, methyltridecane, nonadecane, methylnonadecane, heptadecane, methylheptadecane, pentadecane, methylpentadecane, pentadecene, heptadecene, methylpentadecene, or methylheptadecene.
60 . A cell culture comprising a recombinant microorganism for the production of a hydrocarbon, wherein the recombinant microorganism expresses:
a polynucleotide sequence encoding a decarbonylase;
a polynucleotide sequence encoding a ferredoxin; and
a polynucleotide sequence encoding a ferredoxin reductase.
61 . The cell culture of claim 60 , wherein the hydrocarbon is a C 3 to C 21 alkane or alkene.
62 . A method of producing a hydrocarbon, the method comprising:
culturing a recombinant microorganism in a culture medium comprising a carbon source under conditions effective to produce the hydrocarbon, wherein the recombinant microorganism expresses:
a polynucleotide sequence encoding a decarbonylase;
a polynucleotide sequence encoding a ferredoxin; and
a polynucleotide sequence encoding a ferredoxin reductase.
63 . The method of claim 62 , further comprising purifying the hydrocarbon from the culture medium.
64 . The method of claim 62 , wherein the recombinant microorganism further expresses:
an aldehyde biosynthetic polypeptide that is an acyl-ACP reductase (AAR) that catalyzes the conversion of an acyl-ACP to a fatty aldehyde, or is a carboxylic acid reductase (CAR) that catalyzes the conversion of a fatty acid to a fatty aldehyde; and
optionally, a thioesterase that catalyzes the conversion of an acyl-ACP to a fatty acid.
65 . The method of claim 62 , wherein the hydrocarbon is a C 3 -C 21 alkane or alkene.