Optical signal detection device, gel member, and optical signal detection method
Provided is an optical signal detection device that includes: an objective; a holding member provided between the objective and a sample to hold the sample; and a gel member that fills a space between the objective and the holding member. The gel member has ¼ scale penetration indicating a value of 44 to 111, both inclusive, measured based on a penetration test using a ¼ scale cone of JIS K 2220.
1. An optical signal detection device comprising:
an objective;
a holding member provided between the objective and a sample and configured to hold the sample; and
a gel member that fills a space between the objective and the holding member,
wherein the gel member has ¼ scale penetration indicating a value of 44 to 111, both inclusive, as measured based on a penetration test using a ¼ scale cone of JIS K 2220.
2. The optical signal detection device according to claim 1 , wherein a contact area between the gel member and the objective is larger than a contact area between the gel member and the holding member.
3. The optical signal detection device according to claim 1 , further comprising a revolving nosepiece on which the objective is mounted,
wherein when the revolving nosepiece rotates while the gel member is stuck to the objective, the gel member stuck to the objective is moved out of a light path of the objective along with the objective.
4. The optical signal detection device according to claim 1 , wherein the gel member sticks to the objective in a freely detachable manner.
5. The optical signal detection device according to claim 1 , wherein the gel member has a tapered shape in which a cross-sectional area is reduced from the objective toward the holding member.
6. The optical signal detection device according to claim 5 , wherein the gel member has a truncated cone shape.
7. The optical signal detection device according to claim 1 , wherein the gel member has a convex face formed by a curvature on a holding member side.
8. The optical signal detection device according to claim 1 , wherein a thickness of the gel member is 1.1 times to 1.5 times, both inclusive, of a working distance of the objective.
9. The optical signal detection device according to claim 1 , wherein a refractive index difference between the gel member and the holding member is within +0.1.
10. The optical signal detection device according to claim 1 , wherein a refractive index difference between the gel member and the sample or a medium that covers the sample is within +0.1.
11. A gel member attached to a front end of an objective, the gel member having ¼ scale penetration indicating a value of 44 to 111, both inclusive, as measured based on a penetration test using a ¼ scale cone of JIS K 2220.
12. The gel member according to claim 11 , wherein the gel member sticks to the objective in a freely detachable manner.
13. The gel member according to claim 11 , wherein the gel member has a tapered shape in which a cross-sectional area is reduced toward one direction.
14. The gel member according to claim 13 , wherein the gel member has a truncated cone shape.
15. The gel member according to claim 11 , wherein the gel member has a convex face formed by a curvature.
16. The gel member according to claim 12 , wherein:
a refractive index difference between the gel member and a holding member is within +0.1,
the holding member holds a sample to be observed using the objective between the objective and the sample, and
a space between the objective and the holding member is filled with the gel member.
17. The gel member according to claim 12 , wherein a refractive index difference between the gel member and the sample or a medium that covers a sample to be observed using the objective is within +0.1.
18. The gel member according to claim 12 , wherein a thickness of the gel member is 1.1 times to 1.5 times, both inclusive, of a working distance of the objective.
19. The gel member according to claim 12 , wherein a contact area between the gel member and the objective is larger than a contact area between the gel member and a holding member that holds a sample.
20. An optical signal detection method comprising:
illuminating a sample with light in a state where a space between an objective and a holding member that holds a sample is filled with a gel member having ¼ scale penetration indicating a value of 44 to 111, both inclusive, as measured based on a penetration test using a ¼ scale cone of JIS K 2220; and
detecting, by a photodetector, light from the sample that is incident via the objective.
21. The optical signal detection method according to claim 20 , further comprising:
changing a distance between the objective and the sample in an optical axis direction of the objective; and
every time the distance is changed, repeating illuminating the sample with the light and detecting, by the photodetector, the light from the sample.
22. The optical signal detection device according to claim 1 , wherein the objective is an immersion objective.
23. The gel member according to claim 11 , wherein the objective is an immersion objective.
24. The optical signal detection method according to claim 20 , wherein the objective is an immersion objective.