Recombinant algae having high lipid productivity
The invention provides recombinant algal mutants that have a genetic modification to a gene or nucleic acid sequence encoding a WD40 repeat containing protein or domain. The genetic modification of one or more nucleic acid sequences encoding a WD40 repeat containing protein or domain results in a mutant organism with increased lipid productivity and/or higher biomass productivity (as measured by total organic carbon). The genetic modification can be a gene attenuation or functional deletion. The lipid products of these mutants can be utilized as biofuels or for other specialty chemical products. Methods of making and using the recombinant algal mutants and methods of producing lipids are also disclosed.
1. A recombinant algal organism comprising a deletion, disruption, or inactivation of a gene or nucleic acid sequence encoding a WD40 repeat containing protein having at least 90% sequence identity to SEQ ID NO: 1, wherein the recombinant alga has higher lipid productivity and/or higher biomass productivity versus a corresponding control algal cell not having the genetic modification.
2. The recombinant alga of claim 1 , wherein the recombinant alga is a Chlorophyte alga.
3. The recombinant alga of claim 2 , wherein the alga is of the Class Trebouxiophyceae.
4. The recombinant algal of claim 3 , wherein the gene or nucleic acid sequence encoding a WD40 repeat containing protein comprises a nucleic acid sequence having at least 90% sequence identity to a nucleic acid sequence of SEQ ID NO: 15 or SEQ ID NO: 16.
5. The recombinant alga of claim 3 , wherein the deletion, disruption or inactivation of the gene encoding the WD40 repeat containing protein is a knock out mutation.
6. The recombinant alga of claim 3 , wherein the deletion, disruption, or inactivation of the gene encoding the WD40 repeat containing protein results in an attenuation in the expression of the nucleic acid sequence.
7. The recombinant alga of claim 3 , wherein the deletion, disruption, or inactivation of the gene encoding the WD40 repeat containing protein comprises the deletion of one or more amino acids in a polypeptide sequence of a WD40 repeat containing protein.
8. The recombinant alga of claim 3 , wherein the deletion, disruption, or inactivation of the gene encoding the WD repeat containing protein comprises a frame shift mutation.
9. The recombinant alga of claim 1 , wherein the recombinant alga has at least 30% higher lipid productivity versus a corresponding control alga that does not comprise the genetic modification.
10. The recombinant alga of claim 9 , wherein the recombinant alga has at least 15% higher biomass productivity as measured by total organic carbon versus a control alga that does not comprise the genetic modification.
11. The recombinant alga of claim 1 , wherein the recombinant alga exhibits at least 40 grams per square meter per day of lipid production after 5 days of cultivation.
12. The recombinant alga of claim 1 , wherein the recombinant alga has higher biomass productivity per unit time as measured by production of total organic carbon (TOC).
13. The recombinant alga of claim 12 , wherein the recombinant alga has higher biomass productivity under nitrogen deficient conditions.
14. The recombinant alga of claim 1 , wherein the recombinant alga has higher total organic carbon production under nitrogen deficient conditions.
15. The recombinant alga of claim 3 , wherein the recombinant alga is of a family selected from the group consisting of: Oocystaceae, Chlorellaceae, and Eustigmatophyceae.
16. The recombinant alga of claim 3 , wherein the recombinant alga is of a genus selected from the group consisting of: Chlorella, Parachlorella, Picochlorum, Tetraselmis , and Oocystis.
17. The recombinant alga of claim 16 , wherein the recombinant alga is an alga of the genus Oocystis.
18. A biomass product comprising the recombinant alga of claim 1 .