Red transgenic fluorescent ornamental fish
View Patent ↗The present invention relates to transgenic red ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.
1. A transgenic barb progeny whose genome is obtained from a first transgenic barb, wherein the first transgenic barb exhibits fluorescence and comprises a chromosomally integrated expression cassette encoding a fluorescent protein and a second barb, wherein the first transgenic barb exhibits fluorescence and wherein the chromosomally integrated expression cassette in the first transgenic barb is comprised by the “Red barb 1 transformation event,” sperm comprising the Red barb 1 transformation event having been deposited as ECACC accession no. 16121901, wherein the transgenic barb progeny exhibits fluorescence.
2. The transgenic barb progeny of claim 1 , wherein the first barb is heterozygous for the “Red barb 1 transformation event”.
3. The transgenic barb progeny of claim 1 wherein the first barb is homozygous for the “Red barb 1 transformation event”.
4. The transgenic barb progeny of claim 1 , wherein the second barb is a transgenic barb that exhibits fluorescence.
5. The transgenic barb progeny of claim 4 , wherein the second barb comprises a chromosomally integrated expression cassette encoding a fluorescent protein, the expression cassette comprised by the “Red barb 1 transformation event”, sperm comprising the “Red barb 1 transformation event” having been deposited as ECACC accession no. 1612901.
6. The transgenic barb progeny of claim 5 , wherein the second barb is homozygous for the “Red barb 1 transformation event”.
7. The transgenic barb progeny of claim 5 , wherein the second barb is heterozygous for the “Red barb 1 transformation event”.