Method of adjusting optical apparatus, adjustment support method, optical system, and optical apparatus

1. A method of adjusting an optical apparatus that acquires an image of a sample, the adjustment method comprising the steps of:

moving a focusing section included in the optical apparatus in a direction in which a contrast of a first image of the sample increases; and

adjusting an aberration amount occurring in the optical apparatus in a direction in which a contrast of a second image of the sample increases,

wherein an average value of frequency components of the second image is higher than an average value of frequency components of the first image.

2. The adjustment method according to claim 1 , further comprising the steps of

acquiring the first image by the optical apparatus, and

acquiring the second image by the optical apparatus.

3. The adjustment method according to claim 2 , further comprising the steps of

changing a pupil function of an optical system of the optical apparatus between the step of acquiring the first image and the step of acquiring the second image.

4. The adjustment method according to claim 2 , wherein

the step of acquiring the first image includes the step of modulating at least one of illumination light and observation light by a first modulation element, and

the step of acquiring the second image includes the step of modulating at least one of illumination light and observation light by a second modulation element having a different transmittance distribution from that of the first modulation element.

5. The adjustment method according to claim 4 , wherein the following conditional expression is satisfied:

∫

0

L

⁢

⁢

1

⁢

r

·

f

⁡

(

r

)

⁢

dr

<

∫

0

L

⁢

⁢

2

⁢

r

·

g

⁡

(

r

)

⁢

dr

(

1

)

where f(r) is a transmittance distribution of the first modulation element, g(r) is a transmittance distribution of the second modulation element, r is a distance from an optical axis, L 1 is a radius of an on-axis bundle of rays when the on-axis bundle of rays is incident on the first modulation element, and L 2 is a radius of the on-axis bundle of rays when the on-axis bundle of rays is incident on the second modulation element.

6. The adjustment method according to claim 1 , further comprising the step of

subjecting an image acquired by the optical apparatus to image processing to generate at least one of the first image and the second image.

7. The adjustment method according to claim 6 , wherein

the step of generating at least one of the first image and the second image includes the step of applying a spatial frequency filter to a pupil image obtained by subjecting the image acquired by the optical apparatus to Fourier transformation.

8. The adjustment method according to claim 1 , wherein

the step of adjusting an aberration amount occurring in the optical apparatus includes the step of adjusting a correction ring included in the optical apparatus.

9. An optical system comprising:

an optical apparatus that acquires an image of a sample, the optical apparatus including an objective lens, a focusing section that changes a distance between the objective lens and the sample, and a correction device that corrects an aberration; and

a control device that controls the optical apparatus, wherein

the control device

causes the focusing section to move in a direction in which a contrast of a first image of the sample increases, and

changes setting of the correction device to adjust an aberration amount occurring in the optical apparatus in a direction in which a contrast of a second image of the sample increases, and

an average value of frequency components of the second image is higher than an average value of frequency components of the first image.

10. The optical system according to claim 9 , wherein

the correction device includes a correction ring that corrects a spherical aberration.

11. The optical system according to claim 9 , wherein

the optical apparatus includes a microscope.