Systems and methods for device-independent color measurements for colorimetric sensing
A calibration method and related systems for colorimetric chemical assay and a fluid sensor apparatus using the same are provided. Reference measurements are taken from both a digital camera and a spectrometer. The digital camera is installed in the fluid sensor apparatus. Subsequently, color measurements from the digital camera are then converted and compared to prior spectrometric measurements to perform a colorimetric analysis.
1 . A method for calibrating a digital camera for use in a dialysis system, comprising the steps of:
providing:
the dialysis system including a fluid sensor apparatus; and
the digital camera to be calibrated for use in the dialysis system, wherein the digital camera is configured to receive light projected through the fluid sensor apparatus, when installed to the fluid sensor apparatus;
determining fit parameters for a characteristic equation that inputs a set of two or more image color values associated with a captured image element and outputs a spectral color value, wherein the fit parameters are based on:
images taken at a plurality of different exposure times by the digital camera of each of a plurality of light sources, a set of image color values for each of two or more colors associated with elements in each captured image, and one or more spectral color values associated with each of the plurality of light sources obtained from spectrometer measurements; and
calibrating the digital camera when used in the dialysis system, wherein the calibration is based on at least the determined fit parameters and the characteristic equation.
2 . The method of claim 1 , wherein each of the one or more spectral color values are determined from separate count values of two or more wavelengths measured by the spectrometer.
3 . The method of claim 1 , wherein:
each set of image color values comprise RGB values, and
each spectral color value comprises one or more of the CIELAB color space coordinates, L*, a*, and/or b*.
4 . The method of claim 3 wherein, for each of the plurality of light sources and for each of the RGB values, slope is determined representing a linear relationship between exposure time and image color value, the slope used to determine fit parameters for the characteristic equation.
5 . The method of claim 1 , the camera further including an on-board LED module, the method further comprising the steps of:
for each of a plurality of color reference cards, imaging the card illuminated by the LED module using the digital camera; and
determining brightness and color correction factors on the image color values based on the images of the color references cards.
6 . The method of claim 5 , wherein the color reference cards are filmed through an aqueous solution.
7 . The method of claim 1 , further comprising:
for each of one or more chemical sensor films, measuring the film by the spectrometer through an aqueous solution of a chemical associated with the one or more chemical sensor films, and associating a spectral color value with each chemical sensor film;
the fit parameters are determined based on the associated spectral color values of the one or more chemical sensor films.
8 . The method of claim 7 , the camera further including an onboard LED module, the LED module illuminating each of the one or more chemical sensor films when measuring by the spectrometer.
9 . The method of claim 1 , further comprising:
for each of the one or more chemical sensor films, measuring the film by the spectrometer through a gas including a chemical associated with the sensor film, and associating a spectral color value with each chemical sensor film;
the fit parameters are determined based on the associated spectral color values of the one or more chemical sensor films.