RAPID T-CELL ASSAY FOR SARS-COV-2
Methods for detecting the presence of sever acute respiratory coronavirus 2 (SARS-CoV-2) are provided. The methods comprise incubating a sample comprising T-cells from an individual with one or more SARS-CoV-2 antigens, each antigen having a T-cell epitope, and detecting a change in state of T-cells in the sample. The change in state may be detected by determining the presence or level of a secreted, immune response indicator, such as interferon-gamma (IFN-γ).
1 . A method of detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in an individual, the method comprising:
a. incubating a sample in a container under conditions sufficient for specific recognition by T-cells, wherein the sample comprises T-cells from the individual, wherein the container comprises one or more SARS-CoV-2 antigens, wherein the one or more SARS-CoV-2 antigens are deposited on the interior surface of the container, and wherein the SARS-CoV-2 antigens comprise T-cell epitopes; and
b. detecting a change of state in T-cells in a portion removed from the sample.
2 . The method of claim 1 , wherein the container comprises two or more SARS-CoV-2 antigens.
3 . The method of claim 1 , wherein the one or more SARS-CoV-2 antigens is selected from the group consisting of nucleocapsid (N) protein, membrane (M) protein, envelope (E) protein, and spike (S) protein.
4 . The method of claim 3 , wherein the one or more antigens comprise M protein, E protein, and S protein.
5 . The method of claim 3 , wherein the one or more antigens comprise N protein.
6 . The method of claim 1 , wherein each SARS-CoV-2 antigens comprises between 8 and 50 amino acid residues.
7 . The method of claim 1 , wherein the sample is whole blood, cerebrospinal fluid, synovial fluid, or lymph fluid.
8 . The method of claim 1 , wherein detecting a change in state of T-cells in the sample comprises detecting the presence or level of a T-cell immune response indicator.
9 . The method of claim 8 , wherein the T-cell immune response indicator is selected from the group consisting of IFN-γ, IL-1α, IL-10, IL-2, IL-10, IL-12, IL-17, TNF-α, TNF-β, a granzyme, and a perforin.
10 . The method of claim 9 , wherein the T-cell immune response indicator is IFN-γ.
11 . The method of claim 8 , wherein the presence or level of the T-cell immune response indicator is determined using an antibody that specifically binds a molecule selected from the group consisting of IFN-γ, IL-1α, IL-10, IL-2, IL-10, IL-12, IL-17, TNF-α, TNF-β, a granzyme, and a perforin.
12 . A composition comprising:
a. one or more SARS-CoV-2 peptides, wherein the peptides consist of M, E, S, and/or N peptides;
b. a container for the one or more SARS-COV-2 peptides;
c. T-cells; and
d. an antibody that binds to a T-cell immune response indicator;
wherein each peptide comprises between 8 and 50 amino acids; and
wherein the peptides comprise T-cell epitopes.
13 . The composition of claim 12 , wherein the T-cell immune response indicator is selected from the group consisting of IFN-γ, IL-1α, IL-10, IL-2, IL-10, IL-12, IL-17, TNF-α, TNF-β, a granzyme, and a perforin.
14 . The composition of claim 13 , wherein the T-cell immune response indicator is IFN-γ.
15 . The composition of claim 12 , wherein the container is a channel or depression in a microfluidic device, a well of a microtiter or microwell plate, a microtube, an EPPENDORF® Tube, a microcentrifuge tube, a capillary tube, a test tube, and a blood collection tube.