FOOD PRODUCTS COMPRISING CULTIVATED BOVINE CELLS AND METHODS THEREOF
Provided herein are bovine cells that are adapted to grow in growth medium that contains low-serum or no serum and methods thereof. Also provided are food products made from bovine cells cultivated in vitro and methods for harvesting the cells.
1 . A cell of the genus Bos , wherein the cell is adapted to grow in growth medium that comprises low-serum or no serum.
2 . The cell of the claim 1 , wherein the cell is an immortalized cell (non-tumorigenic cell).
3 . The cell of claim 1 , wherein the serum is calf serum or fetal bovine serum.
4 . (canceled)
5 . (canceled)
6 . The cell of claim 1 , wherein the cell is cultivated in a growth medium that comprises less than 1% serum.
7 . The cell of claim 1 , wherein the cell is cultivated in a growth medium that does not comprise serum.
8 . The cell of claim 1 , wherein the cell is a muscle cell or a fat cell.
9 . The muscle cell of claim 8 , wherein the muscle cell endogenously expresses a cell surface receptor selected from the group consisting of CD29, CD56, and CD82.
10 . The muscle cell of claim 8 , wherein the muscle cell endogenously expresses a transcription factor selected from the group consisting of Pax3, Pax7, Myf5, Mrf4, MyoD, and MyoG.
11 . The muscle cell of claim 8 , wherein the muscle cell does not endogenously express desmin or myosin heavy chain 2 (MyHC2).
12 . (canceled)
13 . (canceled)
14 . A method of cultivating cell of the genus Bos , the method comprising:
adapting the cell to grow in growth medium that comprises low-serum or no serum.
15 . The method of claim 14 , wherein the cell is an immortalized cell (non-tumorigenic cell).
16 . The method of claim 14 , wherein the serum is calf serum or fetal bovine serum.
17 . (canceled)
18 . The method of claim 14 , wherein the growth medium comprises less than 1% serum.
19 . The method of claim 14 , wherein the growth medium comprises no serum.
20 . The method of claim 14 , wherein the cell is a muscle cell or a fat cell.
21 . The method of claim 20 , wherein the muscle cell endogenously expresses a cell surface receptor selected from the group consisting of CD29, CD56, and CD82.
22 . The method of claim 20 , wherein the muscle cell endogenously expresses a transcription factor selected from the group consisting of Pax3, Pax7, Myf5, Mrf4, MyoD, and MyoG.
23 . The method of claim 20 , wherein the muscle cell does not endogenously express desmin or myosin heavy chain 2 (MyHC2).
24 . (canceled)
25 . (canceled)
26 . The method of claim 14 , wherein the growth medium comprises one or more of growth factors, fatty acids, proteins, elements, and small molecules.
27 . (canceled)
28 . (canceled)
29 . The method of claim 26 , wherein the protein comprises transferrin.
30 . The method of claim 26 , wherein the element comprises selenium.
31 . The method of claim 26 , wherein the small molecule is ethanolamine.
32 . (canceled)
33 . The method of claim 14 , wherein the cells are cultivated as a suspension culture.
34 . (canceled)
35 . A method of producing food product, the food product comprising cells of the genus Bos , the method comprising the steps of:
a. culturing the cells in vitro in a growth medium that comprises low or no serum;
b. recovering the cells from the growth medium; and
c. formulating the recovered cells into an edible food product.
36 . (canceled)
37 . The method of claim 35 , wherein the cells are cultivated in a growth medium that does not comprise serum.
38 . (canceled)
39 . (canceled)
40 . (canceled)
41 . (canceled)
42 . (canceled)
43 . (canceled)
44 . (canceled)