Biophotonic compositions, methods, and kits for inhibiting and disrupting biofilms
The present document describes methods of using compositions for inhibiting biofilm formation, or disrupting existing or developing biofilms in a subject, which composition comprises at least one chromophore and a pharmacologically acceptable carrier.
1. A method for preparing a wound for reception of a skin graft in a subject, comprising:
a) topically applying a composition comprising at least one xanthene derivative dye and a pharmaceutically acceptable carrier; and
b) illuminating said composition with actinic light, thereby readying the wound for reception of the skin graft in the subject.
2. The method according to claim 1 , wherein the composition is a biophotonic composition.
3. The method according to claim 1 , wherein the at least one xanthene derivative dye is selected from the group consisting of eosin, erythrosine, fluorescein, phloxine B, rose bengal, and merbromine.
4. The method according to claim 1 , wherein the at least one xanthene derivative dye is eosin Y or eosin B.
5. The method according to claim 1 , wherein the at least one xanthene derivative dye is eosin Y.
6. The method according to claim 1 , wherein the at least one xanthene derivative dye is erythrosine B.
7. The method according to claim 1 , wherein the at least one xanthene derivative dye is present in an amount of from about 0.02% to about 8% by weight of the composition.
8. The method according to claim 1 , wherein the at least one xanthene derivative dye is present in an amount of from about 0.5% by weight of the composition.
9. The method according to claim 1 , wherein the composition further comprises at least one healing factor.
10. The method according to claim 9 , wherein the healing factor is selected from hyaluronic acid, glucosamine, and allantoin.
11. The method according to claim 1 , wherein said composition is illuminated with actinic light for at least one treatment period of from about 1 minute to about 9 minutes per cm 2 of an area to be treated.
12. The method according to claim 1 , wherein said composition is illuminated with actinic light for at least one treatment period of from about 2 minutes to about 8 minutes per cm 2 of an area to be treated.
13. The method according to claim 1 , wherein said composition is illuminated with actinic light for at least two treatment periods, each period followed by a resting interval.
14. The method according to claim 1 , wherein said composition is illuminated with at least two consecutive treatment periods of actinic light wherein each treatment period is from about 1 minute to about 5 minutes per cm 2 of an area to be treated, wherein each treatment period is followed by a resting interval for about 1 minute to about 5 minutes.
15. The method according to claim 1 , further comprising:
a) topically applying the composition to the subject's wound;
b) illuminating the subject's wound with actinic light for a period of from about 1 minute to about 10 minutes;
c) removing the source of actinic light away from the subject's wound for a period of from about 1 minute to about 5 minutes;
d) illuminating the subject's wound with actinic light for a second time period of from about 1 minute to about 10 minutes; and
wherein the first illumination with actinic light activates the composition.
16. The method according to claim 1 , wherein the wound is infected and comprises a biofilm.
17. The method according to claim 16 , wherein the biofilm comprises one of gram-negative bacteria and gram-positive bacteria.
18. The method according to claim 17 , wherein the biofilm comprises a gram-positive bacteria.