METHOD FOR CULTIVATION OF ADHERENT CELLS IN A MULTIPARALLEL BIOREACTOR
Disclosed is a process for growing adherent cells in a containment box of a multi-parallel bioreactor, including: seeding the adherent cells on a carrier held in a culture dish; transferring the adherent cells on the carrier to a containment box of the multi-parallel bioreactor; and growing the adherent cells at a containment box while agitating the media at an impeller speed between 200 rpm to a 1200 rpm.
1 . A process of growing adherent cells in a containment box of a multiparallel bioreactor comprising:
seeding the adherent cells on a carrier held in a culture dish;
transferring the adherent cells on the carrier to a containment box of the multiparallel bioreactor; and
growing the adherent cells at a containment box while agitating the media at an impeller speed between 200 rpm to a 1200 rpm.
2 . A process of claim 1 , wherein the carrier is a PET strip.
3 . A process of claim 1 , wherein the culture dish is a 6-well plate.
4 . A process of optimizing cell growth of adherent cells in a containment box of a multiparallel bioreactor in claim 1 further comprising:
a. harvesting the adherent cells 3-10 days after the transfer of the adherent cells on the carrier to the containment box of the multiparallel bioreactor.
5 . A process of growing adherent cells in a containment box of a multiparallel bioreactor in claim 1 further comprising:
a. infecting the adherent cells on the carrier with at least one virus or virus particles;
b. incubating the adherent cells on the carrier with the virus; and
c. harvesting the virus.
6 . A process of growing adherent cells in a containment box of a multiparallel bioreactor in claim 1 further comprising:
a. treating the cell with at least one vector that produces a biological agent;
b. incubating the adherent cells on the carrier with vector;
c. harvesting the biological agent.
7 . (canceled)
8 . A process of claim 6 wherein the vector is a viral vector selected from a group of a modified vaccinia virus Ankara (MV A), Vascular Stomatitis Virus (VSV), adeno-associated virus (AAV), lentivirus, retrovirus, and adenovirus.
9 . The process of claim 1 wherein the adherent cells are selected from the group consisting of Madin-Darby Canine Kidney Epithelial Cells (MDCK), Madin-Darby Bovine Kidney Epithelial (MDBK) cells, chicken cells or quail cells, PerC6 cells, 3T3 cells, NTCT cells, CHO cells, PK15 cells, MDBK cells, LLC-MK2, MRC-5, HEK293, Hela cells, or a combination or modification thereof.
10 . The process of claim 1 , wherein the adherent cells are Vero cells.
11 . The process of claim 1 , wherein the adherent cells are HEK293 cells.
12 . The process of claim 1 , wherein the impeller speed ranges from 300 rpm to 1000 rpm.
13 . The process of claim 12 , wherein the impeller speed is 300 rpm.
14 . The process of claim 2 , where the growth area of PET strips ranges from 10 cm 2 to 15 cm 2 .
15 . The process of claim 2 , where the growth area of PET strips is 13.9 cm 2 .
16 . The process of claim 2 , wherein the PET strips are made of interwoven fibers.
17 . The process of claim 1 , wherein the adherent cells are grown in a close loop manufacturing system.
18 . The process of claim 1 , wherein the multiparallel bioreactor has at least two containment boxes.
19 . The process of claim 1 , wherein the multiparallel bioreactor has 24 containment boxes.
20 . The process in claim 1 , wherein the multiparallel bioreactor is selected from a group including the AMBR 15, AMBR 250, Solida Biotech parallel bioreactor, and xCubio bioreactor.
21 . The process of claim 1 , wherein the process is used to perform DOE studies or small-scale bioreactor campaigns for production process optimization.
22 . The process in claim 21 , wherein the production processes optimized are cell propagation, virus production, antibody production.