Camptothecine antibody-drug conjugates and methods of use thereof

1. A conjugate of formula (I):

wherein:

Z is CR 10 or N,

R 7 is selected from hydrogen, alkyl, and substituted alkyl;

R 8 and R 9 are each independently selected from hydrogen, alkyl, and substituted alkyl;

each R 10 is independently selected from hydrogen, alkyl, and substituted alkyl;

W is a polypeptide;

L is a linker attached to a compound of formula (II) at R 1 , R 2 , R 3 , R 4 , R 5 or R 6 :

R 1 and R 2 are each independently selected from hydrogen, halogen, hydroxy, amino, substituted amino, alkyl, and substituted alkyl, or R 1 and R 2 are optionally cyclically linked to form a 5 or 6-membered cycloalkyl or heterocyclyl ring;

R 3 and R 4 are each independently selected from hydrogen, halo, hydroxy, amino, substituted amino, alkyl, and substituted alkyl;

R 5 is selected from hydrogen, halogen, hydroxy, amino, substituted amino, alkyl, and substituted alkyl;

R 6 is selected from OH and OC(O)R 11 ; and

R 11 is selected from hydrogen, alkyl, and substituted alkyl,

wherein at least one R 10 is optionally linked to a second compound of formula (II).

2. The conjugate of claim 1 ,

wherein the compound of formula (II) has the structure of formula (IIa):

wherein R 3 is OH and L is attached at R 6 ; or L is attached at R 3 and R 6 is OH; or

wherein the compound of formula (II) has the structure of formula (IIb):

wherein R 1a is selected from H, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, cycloalkyl, substituted cycloalkyl, heterocyclyl, substituted heterocyclyl, carboxyl, carboxyl ester, acyl, and sulfonyl, and L is attached at R 6 ; or L is attached at R 1a and R 6 is OH; or

wherein the compound of formula (II) has the structure of formula (IIc):

wherein R 1b is selected from H, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, cycloalkyl, substituted cycloalkyl, heterocyclyl, substituted heterocyclyl, carboxyl, carboxyl ester, acyl, and sulfonyl, and L is attached at R 6 ; or L is attached at R 1b and R 6 is OH; or

wherein the compound of formula (II) has the structure of formula (IId):

wherein R 2a and R 2b are each independently selected from H, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, cycloalkyl, substituted cycloalkyl, heterocyclyl, substituted heterocyclyl, carboxyl, carboxyl ester, acyl, and sulfonyl, and L is attached at R 6 ; or L is attached at R 2a or R 2b and R 6 is OH; or

wherein the compound of formula (II) has the structure of formula (IIe):

wherein R 2c is selected from alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, cycloalkyl, substituted cycloalkyl, heterocyclyl, substituted heterocyclyl, carboxyl, carboxyl ester, acyl, and sulfonyl, and attachment to L is indicated by the wavy line.

3. The conjugate of claim 1 , wherein L comprises:

-(T 1 -V 1 ) a -(T 2 -V 2 ) b -(T 3 -V 3 ) c -(T 4 -V 4 ) d -(T 5 -V 5 ) e -(T 6 -V 6 ) f -,

wherein

a, b, c, d, e and f are each independently 0 or 1;

T 1 , T 2 , T 3 , T 4 , T 5 and T 6 are each independently selected from a covalent bond, (C 1 -C 12 )alkyl, substituted (C 1 -C 12 )alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, cycloalkyl, substituted cycloalkyl, heterocyclyl, and substituted heterocyclyl, (EDA) w , (PEG) n , (AA) p , —(CR 13 OH) m —, 4-amino-piperidine (4AP), meta-amino-benzyloxy (MABO), meta-amino-benzyloxycarbonyl (MABC), para-amino-benzyloxy (PABO), para-amino-benzyloxycarbonyl (PABC), para-aminobenzyl (PAB), para-amino-benzylamino (PABA), para-amino-phenyl (PAP), para-hydroxy-phenyl (PHP), an acetal group, a hydrazine, a disulfide, and an ester, wherein EDA is an ethylene diamine moiety, PEG is a polyethylene glycol, and AA is an amino acid residue or an amino acid analog, wherein each w is an integer from 1 to 20, each n is an integer from 1 to 30, each p is an integer from 1 to 20, and each m is an integer from 1 to 12;

V 1 , V 2 , V 3 , V 4 , V 5 and V 6 are each independently selected from the group consisting of a covalent bond, —CO—, —NR 15 —, —NR 15 (CH 2 ) q —, —NR 15 (C 6 H 4 )—, —CONR 15 —, —NR 15 CO—, —C(O)O—, —OC(O)—, —O—, —S—, —S(O)—, —SO 2 —, —SO 2 NR 15 —, —NR 15 SO 2 — and —P(O)OH—, wherein each q is an integer from 1 to 6;

each R 13 is independently selected from hydrogen, alkyl, substituted alkyl, aryl, and substituted aryl; and

each R 15 is independently selected from hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, carboxyl, carboxyl ester, acyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, cycloalkyl, substituted cycloalkyl, heterocyclyl, and substituted heterocyclyl.

4. The conjugate of claim 3 , wherein:

T 1 is selected from a (C 1 -C 12 )alkyl and a substituted (C 1 -C 12 )alkyl;

T 2 , T 3 , T 4 , T 5 and T 6 are each independently selected from a covalent bond, (C 1 -C 12 )alkyl, substituted (C 1 -C 12 )alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, cycloalkyl, substituted cycloalkyl, heterocyclyl, and substituted heterocyclyl, (EDA) w , (PEG) n , (AA) p , —(CR 13 OH) m —, 4-amino-piperidine (4AP), MABO, MABC, PABO, PABC, PAB, PABA, PAP, PHP, an acetal group, a hydrazine, and an ester; and

V 1 , V 2 , V 3 , V 4 , V 5 and V 6 are each independently selected from the group consisting of a covalent bond, —CO—, —NR 15 —, —NR 15 (CH 2 ) q —, —NR 15 (C 6 H 4 )—, —CONR 15 —, —NR 15 CO—, —C(O)O—, —OC(O)—, —O—, —S—, —S(O)—, —SO 2 —, —SO 2 NR 15 —, —NR 15 SO 2 —, and —P(O)OH—;

wherein:

(PEG) n is

where n is an integer from 1 to 30;

EDA is an ethylene diamine moiety having the following structure:

where y is an integer from 1 to 6 and r is 0 or 1;

4-amino-piperidine (4AP) is

and

each R 12 is independently selected from hydrogen, an alkyl, a substituted alkyl, a polyethylene glycol moiety, an aryl and a substituted aryl, wherein any two adjacent R 12 groups may be cyclically linked to form a piperazinyl ring.

5. The conjugate of claim 3 , wherein T 1 , T 2 , T 3 , T 4 , T 5 and T 6 are each optionally substituted with a glycoside.

6. The conjugate of claim 3 , wherein MABO, MABC, PABO, PABC, PAB, PABA, PAP and PHP are each optionally substituted with a glycoside.

7. The conjugate of claim 5 , wherein the glycoside is selected from a glucuronide, a galactoside, a glucoside, a mannoside, a fucoside, O-GlcNAc, and O-GalNAc.

8. The conjugate of claim 3 ,

wherein:

T 1 is (C 1 -C 12 )alkyl and V 1 is —CO—;

T 2 is an amino acid analog and V 2 is —NH—;

T 3 is (PEG) n and V 3 is —CO—;

T 4 is AA and V 4 is absent;

T 5 is PABC and V 5 is absent; and

T 6 is EDA and V 6 is —CO—; or

wherein:

T 1 is (C 1 -C 12 )alkyl and V 1 is —CO—;

T 2 is an amino acid analog and V 2 is —NH—;

T 3 is (PEG) n and V 3 is —CO—;

T 4 is AA and V 4 is absent;

T 5 is absent and V 5 is —NR 15 (C 6 H 4 )—; and

T 6 is absent and V 6 is —CO—; or

wherein:

T 1 is (C 1 -C 12 )alkyl and V 1 is —CO—;

T 2 is an amino acid analog and V 2 is —NH—;

T 3 is (PEG) n and V 3 is —CO—;

T 4 is AA and V 4 is absent;

T 5 is PABC and V 5 is —NR 15 —; and

T 6 is (C 1 -C 12 )alkyl and V 6 is —CO—; or

wherein:

T 1 is (C 1 -C 12 )alkyl and V 1 is —CO—;

T 2 is AA and V 2 is absent;

T 3 is PABC and V 3 is absent;

T 4 is EDA and V 4 is —CO—; and

e and f are each 0; or

wherein:

T 1 is (C 1 -C 12 )alkyl and V 1 is —CO—;

T 2 is an amino acid analog and V 2 is —NH—;

T 3 is (PEG) n and V 3 is —CO—;

T 4 is AA and V 4 is absent;

T 5 is PABC and V 5 is absent; and

f is 0; or

wherein:

T 1 is (C 1 -C 12 )alkyl and V 1 is —CO—;

T 2 is AA and V 2 is absent;

T 3 is PABC and V 3 is absent; and

d, e and f are each 0; or

wherein:

T 1 is (C 1 -C 12 )alkyl and V 1 is —CO—;

T 2 is an amino acid analog and V 2 is —NH—;

T 3 is (PEG) n and V 3 is —CO—;

T 4 is AA and V 4 is absent;

T 5 is PABA and V 5 is —CO—; and

T 6 is (C 1 -C 12 )alkyl and V 6 is —SO 2 —; or

wherein:

T 1 is (C 1 -C 12 )alkyl and V 1 is —CONH—;

T 2 is (PEG) n and V 2 is —CO—;

T 3 is AA and V 3 is absent;

T 4 is PABC and V 4 is absent;

e and f are each 0; or

wherein:

T 1 is (C 1 -C 12 )alkyl and V 1 is —CONH—;

T 2 is substituted (C 1 -C 12 )alkyl and V 2 is —CO—;

T 3 is AA and V 3 is absent;

T 4 is PABC and V 4 is absent;

e and f are each 0; or

wherein:

T 1 is (C 1 -C 12 )alkyl and V 1 is —CONH—;

T 2 is (PEG) n and V 2 is —CO—;

T 3 is AA and V 3 is absent;

T 4 is PABC and V 4 is absent;

T 5 is (C 1 -C 12 )alkyl and V 5 is absent;

f is 0; or

wherein:

T 1 is (C 1 -C 12 )alkyl and V 1 is —CO—;

T 2 is 4AP and V 2 is —CO—;

T 3 is (C 1 -C 12 )alkyl and V 3 is —CO—;

T 4 is AA and V 4 is absent;

T 5 is PABC and V 5 is absent;

f is 0; or

wherein:

T 1 is (C 1 -C 12 )alkyl and V 1 is —CO—;

T 2 is 4AP and V 2 is —CO—;

T 3 is (C 1 -C 12 )alkyl and V 3 is —O—;

T 4 is (C 1 -C 12 )alkyl and V 4 is —CO—;

T 5 is AA and V 5 is absent;

T 6 is PABC and V 6 is absent; or

wherein:

T 1 is (C 1 -C 12 )alkyl and V 1 is —CO—;

T 2 is an amino acid analog and V 2 is absent;

T 3 is AA and V 3 is absent;

T 4 is PABC and V 4 is absent;

e and f are each 0; or

wherein:

T 1 is (C 1 -C 12 )alkyl and V 1 is —CONH—;

T 2 is (PEG) n and V 2 is —CONH—;

T 3 is substituted (C 1 -C 12 )alkyl and V 3 is —CO—;

T 4 is AA and V 4 is absent;

T 5 is PABC and V 5 is absent;

f is 0; or

wherein:

T 1 is (C 1 -C 12 )alkyl and V 1 is —CO—;

T 2 is AA and V 2 is —NH—;

T 3 is (PEG) n and V 3 is —CO—;

T 4 is AA and V 4 is absent;

T 5 is PABC and V 5 is absent;

f is 0; or

wherein:

T 1 is (C 1 -C 12 )alkyl and V 1 is —CO—;

T 2 is an amino acid analog and V 2 is —NH—;

T 3 is (PEG) n and V 3 is —CO—;

T 4 is AA and V 4 is absent;

T 5 is PABO and V 5 is absent; and

f is 0; or

wherein:

T 1 is (C 1 -C 12 )alkyl and V 1 is —CO—;

T 2 is an amino acid analog and V 2 is —NH—;

T 3 is (PEG) n and V 3 is —CO—;

T 4 is AA and V 4 is absent;

T 5 is PAP and V 5 is —COO—; and

f is 0; or

wherein:

T 1 is (C 1 -C 12 )alkyl and V 1 is —CONH—;

T 2 is (PEG) n and V 2 is —CO—;

T 3 is AA and V 3 is absent;

T 4 is PAP and V 4 is —COO—; and

e and f are each 0.

9. The conjugate of claim 1 , wherein one R 10 is linked via a second linker, L B , to a second compound of formula (II).

10. The conjugate of claim 9 , wherein L B comprises:

-(T 7 -V 7 ) g -(T 8 -V 8 ) h -(T 9 -V 9 ) i -(T 10 -V 10 ) j -(T 11 -V 11 ) k -(T 12 -V 12 ) l -,

wherein

g, h, i, j, k and l are each independently 0 or 1;

T 7 , T 8 , T 9 , T 10 , T 11 and T 12 are each independently selected from a covalent bond, (C 1 -C 12 )alkyl, substituted (C 1 -C 12 )alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, cycloalkyl, substituted cycloalkyl, heterocyclyl, and substituted heterocyclyl, (EDA) w , (PEG) n , (AA) p , —(CR 13 OH) m —, 4-amino-piperidine (4AP), meta-amino-benzyloxy (MABO), meta-amino-benzyloxycarbonyl (MABC), para-amino-benzyloxy (PABO), para-amino-benzyloxycarbonyl (PABC), para-aminobenzyl (PAB), para-amino-benzylamino (PAB A), para-amino-phenyl (PAP), para-hydroxy-phenyl (PHP), an acetal group, a hydrazine, a disulfide, and an ester, wherein EDA is an ethylene diamine moiety, PEG is a polyethylene glycol, and AA is an amino acid residue or an amino acid analog, wherein each w is an integer from 1 to 20, each n is an integer from 1 to 30, each p is an integer from 1 to 20, and each m is an integer from 1 to 12;

V 7 , V 8 , V 9 , V 10 , V 11 and V 12 are each independently selected from the group consisting of a covalent bond, —CO—, —NR 15 —, —NR 15 (CH 2 ) q —, —NR 15 (C 6 H 4 )—, —CONR 15 —, —NR 15 CO—, —C(O)O—, —OC(O)—, —O—, —S—, —S(O)—, —SO 2 —, —SO 2 NR 15 —, —NR 15 SO 2 — and —P(O)OH—, wherein each q is an integer from 1 to 6;

each R 13 is independently selected from hydrogen, alkyl, substituted alkyl, aryl, and substituted aryl; and

each R 15 is independently selected from hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, carboxyl, carboxyl ester, acyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, cycloalkyl, substituted cycloalkyl, heterocyclyl, and substituted heterocyclyl.

11. The conjugate of claim 10 , wherein T 7 , T 8 , T 9 , T 10 , T 11 and T 12 are each optionally substituted with a glycoside.

12. The conjugate of claim 10 , wherein MABO, MABC, PABO, PABC, PAB, PABA, PAP and PHP are each optionally substituted with a glycoside.

13. The conjugate of claim 11 , wherein the glycoside is selected from a glucuronide, a galactoside, a glucoside, a mannoside, a fucoside, O-GlcNAc, and O-GalNAc.

14. The conjugate of claim 10 ,

wherein:

T 7 is absent and V 7 is —NHCO—;

T 8 is (C 1 -C 12 )alkyl and V 8 is —CO—;

T 9 is AA and V 9 is absent;

T 10 is PABC and V 10 is absent;

T 11 is EDA and V 11 is —CO—; and

l is 0; or

wherein:

T 7 is absent and V 7 is —NHCO—;

T 8 is (C 1 -C 12 )alkyl and V 8 is —CO—;

T 9 is AA and V 9 is absent;

T 10 is PABC and V 10 is absent; and

k and l are each 0; or

wherein:

T 7 is absent and V 7 is —NHCO—;

T 8 is (C 1 -C 12 )alkyl and V 8 is —CO—;

T 9 is an amino acid analog and V 9 is —NH—;

T 10 is (PEG) n and V 10 is —CO—;

T 11 is AA and V 11 is absent; and

T 12 is PABC and V 12 is absent; or

wherein:

T 7 is absent and V 7 is —NHCO—;

T 8 is (C 1 -C 12 )alkyl and V 8 is —CONH—;

T 9 is (PEG) n and V 9 is —CO—;

T 10 is AA and V 10 is absent;

T 11 is PABC and V 11 is absent; and

l is 0; or

wherein:

T 7 is (C 1 -C 12 )alkyl and V 7 is —CONH—;

T 8 is substituted (C 1 -C 12 )alkyl and V 8 is —CO—;

T 9 is AA and V 9 is absent;

T 10 is PABC and V 10 is absent;

k and l are each 0; or

wherein:

T 7 is (C 1 -C 12 )alkyl and V 7 is —CONH—;

T 8 is (PEG) n and V 8 is —CO—;

T 9 is AA and V 9 is absent;

T 10 is PABC and V 10 is absent;

T 11 is (C 1 -C 12 )alkyl and V 11 is absent;

l is 0; or

wherein:

T 7 is (C 1 -C 12 )alkyl and V 7 is —CO—;

T 8 is 4AP and V 8 is —CO—;

T 9 is (C 1 -C 12 )alkyl and V 9 is —CO—;

T 10 is AA and V 10 is absent;

T 11 is PABC and V 11 is absent;

l is 0; or

wherein:

T 7 is (C 1 -C 12 )alkyl and V 7 is —CO—;

T 8 is 4AP and V 8 is —CO—;

T 9 is (C 1 -C 12 )alkyl and V 9 is —O—;

T 10 is (C 1 -C 12 )alkyl and V 10 is —CO—;

T 11 is AA and V 11 is absent;

T 12 is PABC and V 12 is absent; or

wherein:

T 7 is (C 1 -C 12 )alkyl and V 7 is —CO—;

T 8 is an amino acid analog and V 8 is absent;

T 9 is AA and V 9 is absent;

T 10 is PABC and V 10 is absent;

k and l are each 0; or

wherein:

T 7 is (C 1 -C 12 )alkyl and V 7 is —CONH—;

T 8 is (PEG) n and V 8 is —CONH—;

T 9 is substituted (C 1 -C 12 )alkyl and V 9 is —CO—;

T 10 is AA and V 10 is absent;

T 11 is PABC and V 11 is absent;

l is 0; or

wherein:

T 7 is (C 1 -C 12 )alkyl and V 7 is —CO—;

T 8 is AA and V 8 is —NH—;

T 9 is (PEG) n and V 9 is —CO—;

T 10 is AA and V 10 is absent;

T 11 is PABC and V 11 is absent;

l is 0; or

wherein:

T 7 is (C 1 -C 12 )alkyl and V 7 is —CONH—;

T 8 is (PEG) n and V 8 is —CO—;

T 9 is AA and V 9 is absent;

T 10 is PAP and V 10 is —COO—; and

k and l are each 0.

15. The conjugate of claim 1 , wherein the conjugate is selected from:

16. A pharmaceutical composition comprising:

a conjugate of claim 1 ; and

a pharmaceutically-acceptable excipient.

17. A method comprising:

administering to a subject an amount of a conjugate of claim 1 .