Soft tissue protein compositions, methods of making, and uses thereof
Provided herein are soluble soft tissue protein compositions that can contain one or more soft-tissue bioactive factors, methods of making the soluble soft tissue protein compositions, and methods of using the soluble soft tissue protein compositions.
1. A dehydrated soluble soft tissue protein composition comprising a bioactive factor, where the dehydrated soluble soft tissue protein composition is made by a method comprising:
harvesting soft tissue from a donor to obtain harvested soft tissue, where the soft tissue contains soft tissue cells;
heating the harvested soft tissue to at least 20° C.;
lysing the soft tissue cells by osmotic shock without a detergent to obtain a soft tissue cell lysate;
isolating the soft tissue lysate from the soft tissue to yield the soluble soft tissue protein composition; and
dehydrating the soluble soft tissue lysate to obtain a dehydrated soluble soft tissue protein composition.
2. The soluble soft tissue protein composition of claim 1 , further comprising the step of fractionating the soft tissue cell lysate via centrifugation to obtain a soluble soft tissue protein fraction after lysing the soft tissue cells.
3. The soluble soft tissue protein composition of claim 1 , wherein heating the harvested soft tissue occurs at about 20° C. to about 40° C.
4. The soluble soft tissue protein composition of claim 1 , further comprising the step of washing the harvested soft tissue.
5. The soluble soft tissue protein composition of claim 4 , wherein the steps of washing the harvested soft tissue, heating the harvested soft tissue, and lysing soft tissue cells occur simultaneously.
6. The soluble soft tissue protein composition of claim 5 , further comprising the step of applying an amount of the dehydrated soluble soft tissue protein composition in stabilization solution to a scaffold.
7. The soluble soft tissue protein composition of claim 1 , further comprising the step of adding a stabilization solution prior to dehydrating.
8. The soluble soft tissue protein composition of claim 1 , further comprising the step of filtering the soluble soft tissue protein fraction.
9. The soluble soft tissue protein composition of claim 1 , further comprising the step of selectively filtering or isolating the soft tissue cells to obtain a desired cell population prior to lysing the soft tissue cells.
10. The soluble soft tissue protein composition of claim 9 , wherein the step of lysing further comprises lysing the desired cell population.
11. The soluble soft tissue protein composition of claim 9 , wherein the desired cell population is adipocytes.
12. The soluble soft tissue protein composition of claim 1 , further comprising the step of applying an amount of the dehydrated soluble soft tissue protein composition to a scaffold.
13. A method of treating a subject in need thereof comprising implanting or administering a soluble soft tissue protein composition of claim 1 .
14. The method of claim 13 , wherein the subject in need thereof has soft tissue damage.
15. The method of claim 13 , wherein the subject in need thereof is in need of a soft tissue graft.
16. The method of claim 13 , wherein the subject in need thereof is in need of a soft tissue augmentation.