IP Library Granted Patent US 12,466,864
Granted Patent B2
US 12,466,864 · App. 17/593,285 · Granted Nov 11, 2025

Production of large-sized quasidystrophins using overlapping AAV vectors

Inventors: Isabelle Richard (Corbeil Essonnes, FR); William Lostal (Savigny sur Orge, FR)
Assignees: GENETHON; INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE); UNIVERSITE D'EVRY-VAL-D'ESSONNE
C07K14/4708A61K35/76A61P21/00C12N5/0652C12N15/86A61K38/00C12N2750/14143C12N2750/14145C12N2750/14171
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Quick Facts
Patent No.
US 12,466,864
App. No.
17/593,285
Granted
Nov 11, 2025
Kind
B2
Abstract

The present invention concerns a quasidystrophin (QD) having the structure CH1CH2H1R1R2R3H2R8R9 in its N-terminal part and advantageously further comprising the R16 and R17 rod domains, as well as the dual AAV vector system which allows producing it.

Claims (13)

1 . A dual AAV vector system comprising two AAV vectors, wherein

a first AAV vector comprises, between 5′ and 3′ AAV ITRs, a first nucleic acid sequence that encodes a N-terminal part of a quasidystrophin, and

a second AAV vector comprises, between 5′ and 3′ AAV ITRs, a second nucleic acid sequence that encodes a C-terminal part of a quasidystrophin,

wherein the first and second nucleic acid sequences comprise an overlapping region that permits the production by recombination of the quasidystrophin, wherein the quasidystrophin is encoded by a sequence according to SEQ ID NO: 22.

2 . The dual AAV vector system according to claim 1 , wherein the first nucleic acid sequence has the sequence SEQ ID NO: 6 or SEQ ID NO: 23 and the second nucleic acid sequence has the sequence SEQ ID NO: 7.

3 . The dual AAV vector system according to claim 1 , wherein the first nucleic acid sequence has the sequence SEQ ID NO: 8 and the second nucleic acid sequence has the sequence SEQ ID NO: 9.

4 . A cell transduced with the dual AAV vector system according to claim 1 .

5 . The cell of claim 4 , wherein the cell is a muscle cell.

6 . A composition comprising, in a pharmaceutically acceptable carrier, the dual AAV vector system according to claim 1 .

7 . The composition according to claim 6 , wherein the dual AAV vector system is present in a cell into which it has been transduced.

8 . An AAV vector which is the first AAV vector or the second AAV vector of the dual AAV vector system according to claim 1 .

9 . A method of treating muscular dystrophy in a subject in need thereof comprising administering to the subject a therapeutically effective amount of a recombinant AAV comprising the polynucleotide sequence of claim 1 .

10 . The method of claim 9 , wherein the muscular dystrophy is Duchenne muscular dystrophy (DMD).

Assignments (2)
CORRECTIVE ASSIGNMENT TO CORRECT THE ASSIGNEES ADDRESS PREVIOUSLY RECORDED ON REEL 057490 FRAME 0862. ASSIGNOR(S) HEREBY CONFIRMS THE ASSIGNMENT. Recorded Sep 29, 2021
From: RICHARD, ISABELLE; LOSTAL, WILLIAM
To: GENETHON; INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE); UNIVERSITE D'EVRY-VAL-D'ESSONNE
Reel/Frame 057671/0291 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 15, 2021
From: RICHARD, ISABELLE; LOSTAL, WILLIAM
To: GENETHON; INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE); UNIVERSITE D'EVRY-VAL-D'ESSONNE
Reel/Frame 057490/0862 →
Priority Claims (1)
EP 19305377 · Mar 25, 2019 · regional
Continuity (1)
Related Publication 20220204574A1 · Jun 30, 2022
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