Modified plants comprising a polynucleotide comprising a non-cognate promoter operably linked to a coding sequence that encodes a transcription factor
A modified plant is provided. The modified plant includes a polynucleotide including a promoter operably linked to a coding sequence. The coding sequence encodes a transcription factor that has at least 30% sequence identity to one or more of SEQ ID NOs: 27, 204, 10, 19, 1-9, 11-18, 20-26, 28-203, 205, 237, or 242-310. The promoter is non-cognate with respect to the coding sequence. Also provided is a method for producing a modified plant having increased seed yield and/or oil content in comparison to a reference plant by transformation. Also provided is a method for producing a modified plant having increased seed yield and/or oil content in comparison to a reference plant by editing.
1. A modified plant comprising a polynucleotide comprising a promoter operably linked to a coding sequence, wherein:
(a) the coding sequence encodes a transcription factor that is an ortholog of zinc finger CCCH domain-containing protein 54-like protein of Arabidopsis thaliana and has at least 99% sequence identity to SEQ ID NO: 204;
(b) the promoter is non-cognate with respect to the coding sequence; and
(c) the modified plant is Camelina sativa.
2. The modified plant according to claim 1 , wherein the modified plant comprises the polynucleotide based on introduction of the polynucleotide by a transformation procedure.
3. The modified plant according to claim 2 , wherein the non-cognate promoter comprises a constitutive promoter.
4. The modified plant according to claim 2 , wherein the non-cognate promoter comprises a seed-specific promoter.
5. The modified plant according to claim 1 , wherein the coding sequence occurs naturally at a location in the genome of the modified plant, and the modified plant comprises the polynucleotide based on editing of an endogenous promoter that is operably linked to the coding sequence at the location in the genome to make the non-cognate promoter operably linked to the coding sequence at the location in the genome.
6. The modified plant according to claim 5 , wherein the editing increases strength of the non-cognate promoter in comparison to the endogenous promoter.
7. The modified plant according to claim 5 , wherein the editing increases tissue specificity of the non-cognate promoter in comparison to the endogenous promoter.
8. The modified plant according to claim 1 , wherein the modified plant has one or more of the following traits:
(i) a seed yield that is at least 5% higher in comparison to a reference plant not comprising the polynucleotide; or
(ii) a seed oil content that is at least 5% higher in comparison to a reference plant not comprising the polynucleotide.
9. A method for producing a modified plant having increased seed yield and/or oil content in comparison to a reference plant by transformation, the method comprising:
(1) transforming one or more host plants or plant cells with a genetic construct comprising a polynucleotide comprising a promoter operably linked to a coding sequence, wherein:
(a) the coding sequence encodes a transcription factor that is an ortholog of zinc finger CCCH domain-containing protein 54-like protein of Arabidopsis thaliana and has at least 99% sequence identity to SEQ ID NO: 204;
(b) the promoter is non-cognate with respect to the coding sequence; and
(c) the modified plant is Camelina sativa ; and
(2) selecting a transformed plant or plant cell in which the polynucleotide is stably maintained, thereby obtaining the modified plant, wherein the reference plant does not comprise the polynucleotide.
10. The method according to claim 9 , wherein the non-cognate promoter comprises a constitutive promoter.
11. The method according to claim 9 , wherein the non-cognate promoter comprises a seed-specific promoter.
12. A method for producing a modified plant having increased seed yield and/or oil content in comparison to a reference plant by editing, the method comprising:
(1) editing the genome of one or more host plants or plant cells to comprise a polynucleotide comprising a promoter operably linked to a coding sequence, wherein:
(a) the coding sequence encodes a transcription factor that is an ortholog of zinc finger CCCH domain-containing protein 54-like protein of Arabidopsis thaliana and has at least 99% sequence identity to SEQ ID NO: 204;
(b) the promoter is non-cognate with respect to the coding sequence;
(c) the coding sequence occurs naturally at a location in the genome;
(d) the editing is of an endogenous promoter that is operably linked to the coding sequence at the location in the genome to make the non-cognate promoter operably linked to the coding sequence at the location in the genome; and
(e) the modified plant is Camelina sativa ; and
(2) selecting an edited plant or plant cell in which the polynucleotide is stably maintained, thereby obtaining the modified plant, wherein the reference plant does not comprise the polynucleotide.
13. The method according to claim 12 , wherein the editing increases strength of the non-cognate promoter in comparison to the endogenous promoter.
14. The method according to claim 12 , wherein the editing increases tissue specificity of the non-cognate promoter in comparison to the endogenous promoter.
15. The modified plant according to claim 1 , wherein the transcription factor comprises SEQ ID NO: 204.