Subunit vaccine for treatment or prevention of a respiratory tract infection
The present invention relates to modified metapneumovirus (hMPV) F proteins, stabilized in the pre-fusion conformation. It also relates to immunogenic compositions (vaccines) comprising these proteins for preventing and/or treating human subjects against respiratory tract infections.
1. An immunogenic human metapneumovirus (hMPV) modified F protein or fragment thereof, comprising a recombinant single-chain polypeptide lacking a fusion peptide (FP) and comprising an F2 domain, a heterologous peptide linker and an F1 domain, wherein the linker is positioned between the F2 and F1 domains and contains a cysteine residue which forms a disulfide bond with a non-natural cysteine residue present in the F1 domain,
wherein the F2 domain comprises or consists of an amino acid sequence having at least 85%, at least 90%, at least 95% or at least 99% sequence identity to SEQ ID NO: 2,
wherein the F1 domain comprises or consists of an amino acid sequence having at least 85%, at least 90%, at least 95% or at least 99% sequence identity to SEQ ID NO: 3 or to residues 17-388 thereof, and
wherein:
(a) the F1 domain comprises a cysteine residue substituted at position 236 of SEQ ID NO: 3;
(b) the recombinant single-chain polypeptide comprises a cysteine residue substituted at position 338 relative to the amino acid positions of a native hMPV F protein sequence of SEQ ID NO: 1;
(c) the linker comprises a cysteine residue which forms a disulfide bond with a non-natural cysteine residue substituted at position 236 in the F1 domain of SEQ ID NO: 3; and/or
(d) the linker comprises a cysteine residue which forms a disulfide bond with a non-natural cysteine residue substituted at position 338 relative to the amino acid positions of the native hMPV F protein sequence of SEQ ID NO: 1.
2. The immunogenic protein of claim 1 , wherein the linker consists of 1 to 5, preferably 5 amino acids, and wherein the cysteine residue is at position 1 or 3, preferably at position 1 of the linker.
3. The immunogenic protein of claim 1 , wherein the linker comprises a sequence selected from the group consisting of CGAGA (SEQ ID NO: 4), CGAGV (SEQ ID NO: 57, CGAAV (SEQ ID NO: 58), AGCGA (SEQ ID NO: 59), CAAAV (SEQ ID NO: 60), and CAAFV (SEQ ID NO: 61), preferably CGAGA (SEQ ID NO: 4).
4. The immunogenic protein of claim 1 , wherein the single-chain polypeptide further comprises one or more substitution(s) relative to the native hMPV F protein sequence of SEQ ID NO: 1 that stabilize the pre-fusion conformation.
5. The immunogenic protein of claim 1 , wherein the single-chain polypeptide comprises one or more substitution(s) selected from the group consisting of T49M, E51C, E80N, I137W, A147V, A159V, T160F, A161M, K166C, I67L, I177L, F258I, S266D, I480C and/or L481C relative to the native hMPV F protein sequence of SEQ ID NO: 1.
6. The immunogenic protein of claim 1 , wherein the single-chain polypeptide comprises one of the following substitution combinations: N97Q, R102G and G294E; N97Q, R102G, T160F, I177L and G294E; T49M, I67L, E80N, N97Q, R102G, A161M, F258I and G294E; T49M, E51C, I67L, N97Q, R102G, A161M, K166C, S266D, G294E, I480C and L481C; or T49M, N97Q, R102G, I137W, A147V, A159V, A161M, I177L and G294E,
wherein the positions are relative to the amino acid positions of the native hMPV F protein sequence of SEQ ID NO: 1.
7. The immunogenic protein of claim 1 , wherein the single-chain polypeptide comprises a trimerization helper domain (foldon) consisting of the amino acid sequence of SEQ ID NO: 10 or any of SEQ ID NOs 29 to 33, and the amino acid sequence of SEQ ID NO: 34 or SEQ ID NO: 35 upstream of the foldon domain.
8. The immunogenic protein of claim 1 , wherein the single-chain polypeptide comprises or consists of the amino acid sequence any of SEQ ID NOs: 5 to 9 or 24 to 28.
9. An immunogenic composition or vaccine comprising the immunogenic protein of claim 1 .
10. An immunogenic composition or vaccine comprising the immunogenic protein of claim 1 , wherein said immunogenic protein comprises or consists of the amino acid sequence as set forth in SEQ ID NO: 5 or SEQ ID NO: 25 (L7F_A1_23), or a variant thereof having at least 85%, 90%, 95%, 98% or 99% sequence identity thereto.
11. An immunogenic composition or vaccine comprising the immunogenic protein of claim 1 , wherein said immunogenic protein comprises or consists of the amino acid sequence as set forth in as set forth in SEQ ID NO: 6 or SEQ ID NO: 24 (sF_A1_K_L7), or a variant thereof having at least 85%, 90%, 95%, 98% or 99% sequence identity thereto.
12. An immunogenic composition or vaccine comprising the immunogenic protein of claim 1 , wherein said immunogenic protein comprises or consists of the amino acid sequence as set forth in as set forth in SEQ ID NO: 7 or SEQ ID NO: 26 (L7F_A1_31), or a variant thereof having at least 85%, 90%, 95%, 98% or 99% sequence identity thereto.
13. An immunogenic composition or vaccine comprising the immunogenic protein of claim 1 , wherein said immunogenic protein comprises or consists of the amino acid sequence as set forth in SEQ ID NO: 8 or SEQ ID NO: 27 (L7F_A1_33), or a variant thereof having at least 85%, 90%, 95%, 98% or 99% sequence identity thereto.
14. An immunogenic composition or vaccine comprising the immunogenic protein of claim 1 , wherein said immunogenic protein comprises or consists of the amino acid sequence as set forth in SEQ ID NO: 9 or SEQ ID NO: 28 (L7F_A1_4.2), or a variant thereof having at least 85%, 90%, 95%, 98% or 99% sequence identity thereto.
15. A method for generating an immune response against hMPV comprising administering to a human subject an effective amount of the immunogenic protein of claim 1 or immunogenic composition comprising said immunogenic protein.
16. A method for treating or preventing a respiratory tract infection in a human subject comprising administering to the subject an effective amount of the immunogenic protein of claim 1 or immunogenic composition comprising the immunogenic protein of claim 1 .
17. A method for producing the immunogenic protein of claim 1 , wherein said method comprises expressing the immunogenic protein from a nucleic acid molecule of any of the sequences of SEQ ID NOs: 19 to 23 or a nucleic acid molecule having at least 85%, 90%, 95%, 98% or 99% sequence identity to any of SEQ ID NOs: 19 to 23.
18. The method of claim 17 , further comprising combining the expressed protein with a pharmaceutically acceptable carrier and/or excipient.
19. The immunogenic composition or vaccine of claim 9 , further comprising a pharmaceutically acceptable carrier and/or excipient.
20. The immunogenic composition or vaccine of claim 9 , further comprising an adjuvant.