CONDITIONED MEDIUM AND METHODS OF USE THEREOF
The present invention is directed to a methods for the promotion of autophagy and/or proteasome activation in a subject by administering a cell culture conditioned media (CCM) composition. The present invention also provides methods for reducing symptoms of aging and treating diseases or conditions including Alzheimer's disease and lung fibrosis.
1 . A method of promoting autophagy and/or proteasome activation in a subject comprising administering to the subject a cell culture conditioned medium (CCM) composition, thereby promoting autophagy and/or proteasome activation.
2 . The method of claim 1 , wherein the CCM composition is produced by a method comprising:
culturing cells in a suitable growth medium, wherein the cells produce and secrete a cell culture conditioned medium (CCM) composition.
3 . The method of claim 2 , wherein the cells are cultured under hypoxic or normoxic conditions.
4 . The method of claim 2 , wherein the hypoxic conditions comprise 1-5% O 2 .
5 . The method of claim 2 , wherein the cells are fibroblast cells.
6 . The method of claim 1 , wherein the cells are grown under one, two or three dimensional conditions.
7 . The method of claim 6 , wherein the cells are grown in a monolayer, on beads or on mesh.
8 . The method of claim 1 , wherein the CCM composition is administered topical, oral or intravenous administration.
9 . The method of claim 1 , wherein expression of ATG5, ATG7, ATG12, BECN1, MAP1LC3, POMP, PSMB5, PSMB6 or a combination thereof is increased following administration of the CCM.
10 . A method of reducing symptoms of aging in a subject comprising administering to the subject a cell culture conditioned medium (CCM) composition, thereby reducing the symptoms of aging.
11 . The method of claim 10 , wherein the CCM composition is produced by a method comprising:
culturing cells in a suitable growth medium, wherein the cells produce and secrete a cell culture conditioned medium (CCM) composition.
12 . The method of claim 10 , wherein symptoms of aging are selected from the group consisting of fine lines, wrinkles, dark spots, dry skin, loosening of the skin, and dulling of skin tone.
13 . The method of claim 10 , wherein the CCM composition is administered topically.
14 . A method of preventing, treating and/or ameliorating symptoms of a disease or condition in a subject comprising administering to the subject a cell culture conditioned medium (CCM) composition, thereby preventing, treating and/or ameliorating symptoms of a disease or condition.
15 . The method of claim 14 , wherein the CCM composition is produced by a method comprising:
culturing cells in a suitable growth medium, wherein the cells produce and secrete a cell culture conditioned medium (CCM) composition.
16 . The method of claim 14 , wherein the disease or condition is Alzheimer's disease, lung fibrosis, Huntington's disease, Amyotrophic lateral sclerosis, muscular dystrophies characterized by downregulated proteasome functions and other diseases of aging caused by diminished autophagy and proteasome activity.
17 . The method of claim 14 , wherein the goal is to prolong general or specific tissue and organ health or a youthful appearance.
18 . The method of claim 8 , wherein the CCM composition is administered by topical, oral, nasal, alveolar lavage, inhalation, intravenous or intracranial administration.
19 . The method of claim 8 , wherein the CCM composition is administered in a nano-container conjugated to a targeting moiety for specific tissue delivery.
20 . The method of claim 19 , where the targeting moiety for specific tissue delivery is an aptamer, antibody, antibody fragment, peptide or affinity ligand that recognizes a tissue specific surface or internalization biomarker.