Photoactivated blood products and methods and apparatus for fabrication and use of same
Embodiments provide methods and apparatus for fabricating blood products, such as platelet-rich plasma, containing elevated concentrations of growth factors such as platelet derived growth factor. The platelet-rich plasma can be autologous, and the concentration of growth factors (e.g., platelet derived growth factor) is elevated relative to other samples isolated from the same subject. The platelet-rich plasma can be used to promote tissue regeneration, including wound healing, joint repair, hair growth, and the like. The compositions can be combined with stem cells and used to treat disorders otherwise treated with stem cells. The growth factors present in the samples can direct the differentiation of the stem cells.
1. A method of making a light-irradiated plasma product from blood, the method comprising:
(a) processing a quantity of whole blood by controlling a spectral content of light applied to the quantity of whole blood such that a concentration of a growth factor in the quantity of whole blood is increased, wherein controlling the spectral content of the applied light comprises providing light in a wavelength range of from 660 nm to 1500 nm; and
(b) extracting a plasma product including the growth factor from the processed quantity of whole blood.
2. The method of claim 1 , wherein controlling the spectral content of the applied light comprises providing light in a wavelength range from 600 nm to 900 nm and wherein the growth factor comprises a platelet-derived growth factor (PDGF).
3. The method of claim 1 , further comprising controlling a radiant energy of the applied light.
4. The method of claim 3 , wherein controlling the radiant energy of the applied light comprises controlling an exposure time of the applied light.
5. The method of claim 1 , wherein controlling the spectral content of the applied light comprises irradiating the quantity of whole blood with a solid-state light source.
6. The method of claim 5 , wherein the solid-state light source comprises a LED light source.
7. The method of claim 6 , wherein the LED light source has a maximum spectral power in a wavelength range from 600 nm to 1500 nm.
8. The method of claim 1 , wherein controlling the spectral content of the applied light further comprises irradiating a transparent vessel containing the quantity of whole blood with a spectrally-selective light source while the transparent vessel is housed in an enclosure that blocks exposure of the vessel to ambient light.
9. The method of claim 8 , wherein the spectrally-selective light source comprises an LED light source.
10. The method of claim 1 , further comprising administering a therapeutically effective amount of a composition including the plasma product to tissue of a subject in need thereof to promote tissue regeneration.
11. The method of claim 10 , wherein a concentration of the growth factor in the composition is at least 50% higher than a concentration of the growth factor in an un-irradiated sample of platelet-rich plasma isolated from a source of the quantity of whole blood.
12. The method of claim 10 , wherein the tissue regeneration comprises increased hair growth.
13. The method of claim 10 , wherein the tissue regeneration comprises reduction of skin wrinkles.
14. The method of claim 3 , wherein controlling the radiant energy of the applied light includes a radiant energy of at least 1 J/cm 2 .
15. The method of claim 3 , wherein controlling the radiant energy of the applied light includes a radiant energy of at least 10 J/cm 2 .
16. The method of claim 10 , wherein the quantity of whole blood is derived from the subject.