METHOD OF DIGITAL MULTIPLEX DETECTION AND/OR QUANTIFICATION OF BIOMOLECULES AND USE THEREOF

1 . A digital multiplex method for detecting and/or quantifying multiple target biomolecules in a sample, comprising the following steps:

a) functionalizing a suspension of particles with one or more oligonucleotides selected from a first oligonucleotide which is a conversion oligonucleotide (cT), a second oligonucleotide which is a reporting oligonucleotide (rT), a third oligonucleotide which is an amplification oligonucleotide (aT), and a fourth oligonucleotide which is a leak absorption oligonucleotide (pT);

b) adding to the particles functionalized in step a) barcodes allowing the discrimination of the particles targeting multiple biomolecules;

c) contacting the particles obtained in step b) with a tested sample to capture the multiple target biomolecules;

d) resuspending the particles having captured or not the target biomolecules in a common amplification mixture including a buffer, enzymes, deoxy-nucleoside triphosphate (dNTPs) and optionally oligonucleotides;

e) separating the particles in the suspension obtained in step d) from each other so that each particle can react independently;

f) incubating the particles at a constant temperature so that each target biomolecule triggers an amplification reaction which generates an amplification signal on the particle carrying the target, and

g) detecting and/or measuring the signals of the particles including the barcode signal and the amplification signal of each particle.

2 . The digital multiplex method of claim 1 , wherein the functionalization of the suspension of particles in step a) is performed with the first oligonucleotide and with the second oligonucleotide, and

wherein the third oligonucleotide and the fourth oligonucleotide are added in the amplification mixture in step d).

3 . The digital multiplex method of claim 1 , wherein steps a) and b) are performed concomitantly.

4 . The digital multiplex method of claim 1 , further comprising a step e1) of recovering the particles.

5 . The digital multiplex method according claim 1 , wherein the enzymes used in step d) are selected from the group consisting of polymerase, nicking enzyme or restriction enzyme, and exonuclease.

6 . The digital multiplex method according to claim 1 , wherein the suspension obtained in step d) is separated in step e) into droplets.

7 . The digital multiplex method according to claim 1 , wherein the constant temperature in step f) is between 30 and 55° C.

8 . The digital multiplex method according to claim 1 , wherein the functionalized particles are selected from porous or non-porous particles and hydrogel particles having a size between 10 nm and 500 μm.

9 . The digital multiplex method according claim 1 , wherein the step g) of detecting and/or measuring said barcode signal comprises detecting and/or measuring the barcode signal for each particle associated to the target biomolecule and the signal resulting from the amplification.

10 . The digital multiplex method of claim 1 , wherein the target biomolecules are of the same kind or of different kind, said biomolecules being nucleic acids or proteins.

11 . The digital multiplex method according to claim 10 , wherein the target biomolecules are nucleic acids selected from the group consisting of DNAs, cDNAs, RNAs, mRNAs, and microRNAs.

12 . The digital multiplex method according to claim 1 , wherein the target biomolecule is used as a biomarker.

13 . An in vitro method for diagnosis of a disease selected from the group consisting of cancer, neuronal diseases, cardiovascular diseases, inflammatory diseases, autoimmune diseases, diseases due to a viral or bacterial infection, skin diseases, skeletal muscle diseases, dental diseases, and prenatal diseases comprising the use of the digital multiplex method according to claim 1 .

14 . An in vitro method for agro diagnosis of a disease selected from the group comprising:

diseases caused by biotic stress, or

diseases caused by abiotic stress,

said method comprising the use of the multiplex digital method according to claim 1 .

15 . A kit for detecting and/or quantifying multiple target biomolecules comprising:

a) a suspension of particles functionalized with a one or more oligonucleotides selected from a first oligonucleotide which is a conversion oligonucleotide (cT), a second oligonucleotide which is a reporting oligonucleotide (rT), a third oligonucleotide which is an amplification oligonucleotide (aT), and a forth oligonucleotide which is a leak absorption oligonucleotide (pT), to which particles are added different barcodes allowing the discrimination of the particles targeting different biomolecules;

b) a mixture of enzymes, and

c) a separating agent.

16 . The digital multiplex method of claim 1 , wherein the particles are microparticles.

17 . The digital multiplex method according to claim 6 , wherein the droplets are water-in-oil emulsion droplets having a size of droplet is comprised between 0.001 and 100 pL.

18 . The digital multiplex method according to claim 9 , wherein said barcode signal is a fluorescence signal.

19 . The in vitro method for agro diagnosis according to claim 14 , wherein:

the diseases caused by biotic stress are from infectious and/or parasitic origin, or

the diseases caused by abiotic stress are caused by nutritional deficiencies and/or unfavorable environment,

20 . The kit according to claim 15 , wherein the particles are microparticles and the enzymes of said mixture are selected from the group consisting of polymerase, nicking enzyme or restriction enzyme, and exonuclease.