Method for detecting vitamin D in blood by using LDI-MS, and device for same
View Patent ↗A method of detecting vitamin D in blood using laser desorption/ionization mass spectrometry (LDI-MS) and an apparatus therefor according to the present invention are not complicated in a measurement, do not require a number of measurement steps, and allow for easy measurement and collection of results in real time with a quick analysis. In addition, precise analysis may be performed even at a lower concentration of a sample, such that sensitivity and precision are excellent, various subtypes of vitamin D may be simultaneously detected, a throughput is high, and structural analysis and quantitative analysis of vitamin D that has undergone a metabolic process in blood may be accurately performed without a matrix interference.
1. A method of detecting vitamin D in blood using laser desorption/ionization mass spectrometry (LDI-MS), the method comprising:
adding an internal standard to a sample comprising blood;
loading the sample on a tungsten ditelluride layer of an LDI-MS sample loading array including a substrate layer and the tungsten ditelluride layer stacked on the substrate layer;
acquiring an LDI-MS spectrum of the sample; and
setting a ratio of a peak intensity of the internal standard to a peak intensity of vitamin D identified in the LDI-MS spectrum on a y-axis and setting a concentration of vitamin D in the sample on an x-axis to obtain a calibration curve.
2. The method of claim 1 , wherein the tungsten ditelluride layer includes a plurality of tungsten ditelluride nanoflake particles in contact with the substrate layer.
3. The method of claim 2 , wherein an average thickness of the tungsten ditelluride nanoflake particles is 2 to 15 nm.
4. The method of claim 2 , wherein the tungsten ditelluride nanoflake particles in the tungsten ditelluride layer is present in an amount of 0.0001 to 100 mg/cm 2 per unit area.
5. The method of claim 2 , wherein the tungsten ditelluride layer is produced by spotting a solution in which tungsten ditelluride nanoflake particles are dispersed on the substrate layer and then evaporating a dispersion medium.
6. The method of claim 5 , wherein the tungsten ditelluride nanoflake particles in the solution are included in an amount of 0.001 to 10 wt %.
7. The method of claim 6 , wherein the solution is spotted in the same region in an amount of 0.001 to 100 μl (25° C., 1 atm) two times or more.
8. The method of claim 1 , a coefficient of determination (R 2 ) in the calibration curve is 0.9 or more.
9. The method of claim 1 , wherein an average thickness of the tungsten ditelluride layer is 0.001 to 500 μm.
10. The method of claim 1 , wherein the substrate layer includes one or two or more selected from aluminum, copper, iron, nickel, zinc, chromium, silver, and silicon.