COMBINATION VACCINE FOR INTRADERMAL ADMINISTRATION
The present invention relates to the field of veterinary vaccinology, namely to combination vaccines for swine. In particular the invention relates to a combination vaccine for protection against a pathogenic infection with porcine circo vims type 2 (PCV2) and Mycoplasma hyopneumoniae (Mhyo) comprising non-replicating immunogen of PCV2 and non-replicating immunogen of Mhyo. The vaccine is characterized in that it is an oil-in-water emulsion comprising squalane, vitamin E-acetate and silica. In another embodiment, the invention relates to a combination vaccine for protection against a pathogenic infection with PCV2 and Mhyo by intradermal administration.
1 . A combination vaccine comprising a non-replicating immunogen of porcine circo virus type 2 and a non-replicating immunogen of Mycoplasma hyopneumoniae , characterized in that the combination vaccine is an oil-in-water emulsion comprising squalane, vitamin E-acetate and silica.
2 . The combination vaccine according to claim 1 , characterized in that it comprises squalane in an amount of 1 to 15% w/v.
3 . The combination vaccine according to claim 1 , characterized in that it comprises vitamin E-acetate in an amount of 2 to 20% w/v.
4 . The combination vaccine according to claim 1 , characterized in that it comprises an emulsifier with an HLB value of 8 to 20.
5 . The combination vaccine according to claim 4 , characterized in that the emulsifier is polysorbate 80.
6 . The combination vaccine according to claim 4 , characterized in that the emulsifier is present in an amount of 0.5 to 10% w/v.
7 . The combination vaccine according to claim 1 , characterized in that it comprises silica in an amount of 0.02 to 2% w/v.
8 . The combination vaccine according to claim 1 , characterised in that the non-replicating immunogen of porcine circo virus type 2 is recombinantly expressed protein encoded by the ORF2 gene of porcine circo virus type 2.
9 . The combination vaccine according to claim 1 , characterised in that the non-replicating immunogen of Mycoplasma hyopneumoniae comprises killed whole Mycoplasma hyopneumoniae.
10 - 12 . (canceled)
13 . A method of prophylactically treating an animal against an infection with porcine circo virus type 2 and an infection with Mycoplasma hyopneumoniae by administering into the dermis of the animal the combination vaccine according to claim 1 .
14 . An adjuvant composition for formulating a non-live vaccine, characterized in that the composition is an oil-in-water emulsion comprising squalane, vitamin E-acetate and silica.