PIPETTABLE REPLACEMENT FLUID FOR MIMICKING PIPETTABILITY OF PCR MASTERMIX AND RELATED METHOD
A replacement fluid suitable to replace a PCR Mastermix, wherein the replacement fluid is different from the PCR Mastermix. The replacement fluid has pipetting characteristics substantially equivalent to the PCR Mastermix, wherein for a desired pipetted volume of the target fluid delivered by a pipette, a pipetted volume of the replacement fluid delivered by the pipette is substantially equivalent to the desired pipetted volume of the PCR Mastermix. The replacement fluid is a primary equivalent fluid having substantially similar pipetting characteristics. One or more additives may be added to the primary equivalent fluid. The one or more additives may include pipettability modifying additives and non pipettability modifying additives.
1 . A replacement fluid suitable to replace PCR Mastermix, wherein the replacement fluid is different from the PCR Mastermix, the replacement fluid comprising:
a fluid having pipetting characteristics substantially equivalent to PCR Mastermix, wherein for a desired pipetted volume of the PCR Mastermix delivered by a pipette, a pipetted volume of the replacement fluid delivered by the pipette is substantially similar to the desired pipetted volume of the PCR Mastermix.
2 . The replacement fluid as claimed in claim 1 , wherein the pipetted volume of the replacement fluid is within about +/−20% of the desired pipetted volume of the PCT Mastermix.
3 . The replacement fluid as claimed in claim 1 , wherein the replacement fluid has rheological and other properties substantially equivalent to rheological properties of PCR Mastermix.
4 . The replacement fluid as claimed in claim 3 , wherein the rheological properties and other properties include viscosity, shear rate, surface tension, and contact angle.
5 . The replacement fluid as claimed in claim 4 , wherein the replacement fluid includes a primary equivalent fluid and one or more additives.
6 . The replacement fluid as claimed in claim 5 , wherein the one or more additives include one or more pipetting-modifying additives selected to modify one or more of the rheological and other properties of the primary equivalent fluid.
7 . The replacement fluid as claimed in claim 6 , wherein the one or more pipetting-modifying additives are selected from the group consisting of water, a water-soluble polymer, buffering salt, glycerol, sucrose, protein, lipid, and surfactant.
8 . The replacement fluid as claimed in claim 5 , wherein the one or more additives include one or more non-pipetting-modifying additives selected not to modify one or more of the rheological and other properties of the primary equivalent fluid.
9 . The replacement fluid as claimed in claim 8 , wherein the one or more non-pipetting-modifying additives are selected from the group consisting of stabilizing buffer, chelator, and preservative agent.
10 . The replacement fluid as claimed in claim 7 , wherein the primary equivalent fluid is a mix of water and 20% glycerol and the pipetting-modifying additive is a surfactant.
11 . The replacement fluid as claimed in claim 10 , wherein the surfactant is a nonionic surfactant.
12 . The replacement fluid as claimed in claim 11 , wherein the nonionic surfactant is octylphenol ethoxylate.
13 . The replacement fluid as claimed in claim 1 , further comprising one or more analysis-aiding components, wherein the one or more analysis-aiding components are selected to enable analysis of the replacement fluid as though it were the PCR Mastermix.
14 . The replacement fluid as claimed in claim 31 , wherein the one or more analysis-aiding components are one or more absorbance or fluorescence dyes selected to enable spectroscopic analysis of the replacement fluid.