METHODS AND COMPOSITIONS FOR WHOLE TRANSCRIPTOME AMPLIFICATION
The disclosure provides for methods, compositions, systems, devices, and kits for whole transcriptome amplification using stochastic barcodes.
1 . A kit, comprising:
a plurality of solid supports, wherein each of the solid support comprises a plurality of stochastic barcodes each comprising a first universal label;
an adaptor comprising a second universal label; and
an enzyme, wherein the enzyme is a ligase or a transposase.
2 . The kit of claim 1 , wherein said enzyme is a ligase.
3 . The kit of claim 2 , wherein the ligase is a T4 DNA ligase.
4 . The kit of claim 1 , wherein said enzyme is a transposase.
5 . The kit of claim 4 , wherein said transposase is a Tn5 transposase, or a hyperactive derivative thereof.
6 . The kit of claim 1 , wherein said plurality of solid supports comprises one or more discrete particles of plastic, ceramic, metal, or polymeric material.
7 . The kit of claim 6 , wherein the one or more discrete particles are beads.
8 . The kit of claim 7 , wherein the one or more beads are hydrogel beads.
9 . The kit of claim 1 , further comprising a microwell array.
10 . The kit of claim 1 , wherein said second universal label is at most 99% identical to said first universal label.
11 . The kit of claim 1 , wherein the stochastic barcodes are covalently immobilized to the solid support.
12 . The kit of claim 1 , wherein the stochastic barcodes are non-covalently immobilized to the solid support.