Analyte depletion for sensor equilibration
View Patent ↗Methods include treating a portion of a sample composition to be tested for presence of an analyte by depleting or blocking the target analyte. The treated composition may be used to equilibrate an acoustic wave sensor prior to exposing the sensor to the untreated sample composition for analysis. By using the treated sample composition, in which the analyte is depleted or blocked, to equilibrate the sensor, the sensor may be equilibrated with a composition having a similar viscosity and non-specific binding characteristics to the untreated sample composition, which should result in improved accuracy when analyzing the analyte in the untreated sample composition.
1. A method comprising:
providing a composition to be tested for the presence of an analyte;
contacting a first portion of the composition with a material configured to remove or block the analyte from the composition;
flowing the first portion of the composition over a surface of an acoustic wave sensor, wherein an analyte capture ligand is bound to the surface of the acoustic wave sensor; and
flowing a second portion of the composition over the surface of the acoustic wave sensor, wherein the second portion of the composition has not been contacted with the material configured to remove or block the analyte.
2. The method of claim 1 , further comprising driving the acoustic wave sensor into oscillating motion and monitoring an output wave propagation characteristic from the acoustic wave sensor while the first portion of the composition is flowing over the surface of the acoustic wave sensor.
3. The method of claim 2 , further comprising:
flowing the second portion of the composition over the surface of the acoustic wave sensor after the first portion of the composition has been flowed over the surface of the acoustic wave sensor;
monitoring the wave propagation characteristic of the acoustic wave sensor while the second portion of the composition is flowing over the surface of the acoustic wave sensor; and
comparing the monitored wave propagation characteristic when the second portion of the composition is flowed over the surface of the acoustic wave sensor to the monitored wave propagation characteristic when the first portion of the composition is flowed over the surface of the acoustic wave sensor.
4. The method of claim 3 , further comprising correlating a change in the monitored wave propagation characteristic when the second portion of the composition is flowed over the surface of the acoustic wave sensor relative to the monitored wave propagation characteristic when the first portion of the composition is flowed over the surface of the acoustic wave sensor to concentration of analyte in the second portion of the composition.
5. The method of claim 1 , wherein the material configured to remove or block the analyte from the composition comprises the analyte capture ligand.
6. The method of claim 1 , wherein contacting the first portion of the composition with the material configured to remove or block the analyte from the composition comprises flowing the first portion of the composition over or through the material.
7. The method of claim 6 , wherein flowing the first portion of the composition over or through the material comprises flowing the first portion of the composition through a channel, wherein the material is disposed on a surface of the channel or disposed in the channel.
8. The method of claim 1 , further comprising placing the first portion of the composition in a chamber.
9. The method of claim 8 , wherein the chamber contains the material configured to remove or block the analyte from the composition when the first portion of the composition is placed in the chamber.
10. The method of claim 9 , wherein the placing of the first portion of the composition in the chamber occurs when the composition is introduced into a port of a device configured to determine the presence of an analyte in the composition.
11. The method of claim 1 , further comprising flowing the first portion of the composition over the surface of the acoustic wave sensor after the first portion has contacted the material configured to remove or block the analyte from the composition.
12. A method comprising:
providing a composition to be tested for the presence of an analyte;
flowing a first portion of the composition over a surface of an acoustic wave sensor,
wherein an analyte capture ligand is bound to the surface of the acoustic wave sensor; and
wherein the first portion of the composition has previously been contacted with a material configured to remove or block the analyte from the composition; and
flowing a second portion of the composition over the surface of the acoustic wave sensor, wherein the second portion of the composition has not been contacted with the material configured to remove or block the analyte.
13. The method of claim 12 , wherein the first portion of the composition is introduced through a first port of a device configured to determine the presence of an analyte in the composition.
14. The method of claim 12 , wherein the second portion of the composition is introduced through a second port of a device configured to determine the presence of an analyte in the composition.
15. The method of claim 12 , further comprising:
driving the acoustic wave sensor into oscillating motion and monitoring an output wave propagation characteristic from the acoustic wave sensor while the first portion of the composition is flowing over the surface of the acoustic wave sensor;
flowing the second portion of the composition over the surface of the acoustic wave sensor after the first portion of the composition has been flowed over the surface of the acoustic wave sensor;
monitoring the wave propagation characteristic of the acoustic wave sensor while the second portion of the composition is flowing over the surface of the acoustic wave sensor; and
comparing the monitored wave propagation characteristic when the second portion of the composition is flowed over the surface of the acoustic wave sensor to the monitored wave propagation characteristic when the first portion of the composition is flowed over the surface of the acoustic wave sensor.
16. A method for equilibration of an acoustic wave sensor for testing the presence of an analyte, comprising:
providing a composition to be tested for the presence of the analyte;
contacting a first portion of the composition with a material configured to remove or block the analyte from the composition; and
flowing the first portion of the composition over a surface of the acoustic wave sensor to equilibrate the acoustic wave sensor for testing the presence of an analyte in a second portion of the composition,
wherein an analyte capture ligand is bound to the surface of the acoustic wave sensor; and
wherein the second portion of the composition has not been contacted with a material configured to remove or block the analyte.
17. The method of claim 16 , wherein the composition is introduced into a first sample compartment of a device configured to determine the presence of an analyte in the composition, and wherein the first portion of the composition is diverted to a first sub-compartment of the device.
18. The method of claim 17 , wherein the first sub-compartment comprises the material configured to remove or block the analyte from the composition.
19. The method of claim 17 , wherein contacting the first portion of the composition with the material configured to remove or block the analyte from the composition comprises flowing the first portion of the composition over or through the material.
20. The method of claim 19 , wherein flowing the first portion of the composition over or through the material comprises flowing the first portion of the composition through a channel that is downstream from the first sub-compartment, wherein the material is disposed on a surface of the channel or disposed in the channel.