CELL SUSPENSION COMPOSITION WITH THERAPEUTIC POTENTIAL AND RELATED METHODS AND SYSTEMS FOR IDENTIFYING SAME
Systems and methods for identifying a cell suspension with therapeutic potential for skin regeneration and related compositions are disclosed herein. In some variations, a method may include receiving a cell suspension that comprises a population of viable cells and non-viable cells, then measuring a value indicative of at least one characteristic of the cell suspension, such as but not limited to one or more of total cell count, total cell viability, cell viability percentage, and median live cell diameter. A cell suspension composition having therapeutic potential may comprise a cell suspension met certain thresholds relating to total cell count, total viable cell count, cell viability percentage, and median live cell diameter.
1 . A method for identifying a cell suspension with therapeutic potential for skin regeneration, comprising:
receiving a cell suspension, wherein the cell suspension is prepared from a skin sample and comprises a population of viable cells and non-viable cells;
measuring a value indicative of at least one characteristic of the cell suspension, wherein the at least one characteristic relates to one or more of total cell count, cell viability percentage, total viable cell count, and median live cell diameter; and
identifying the cell suspension as having therapeutic potential for skin regeneration based on a comparison between the value indicative of the at least one characteristic of the cell suspension and a predetermined threshold.
2 . The method of claim 1 , wherein the at least one characteristic is total cell count, and the value relating to total cell count is at least about 550,000 cells/ml.
3 . The method of claim 1 , wherein the at least one characteristic is cell viability percentage and the value relating to cell viability percentage is at least 30% viable cells.
4 . The method of claim 1 , wherein the at least one characteristic is total viable cell count and the value relating to total viable cell count is at least 300,000 viable cells/ml.
5 . The method of claim 1 , wherein the at least one characteristic is median live cell diameter and the value relating to median live cell diameter is a median live cell diameter of least 9 micrometers.
6 . The method of claim 1 , further comprising the steps of:
removing all cells from the cell suspension.
7 . A system for identifying a cell suspension with therapeutic potential for skin regeneration, comprising:
a cell detection, measurement, and/or counting device configured to receive a cell suspension, wherein the cell suspension is prepared from a skin sample and comprises a population of viable cells and non-viable cells; and
a processor coupled to the cell detection, measurement, and/or counting device, the processor configured to:
i) measure a value indicative of at least one characteristic of the cell suspension received by the cell detection, measurement, and/or counting device wherein the characteristic relates to one or more of total cell count, cell viability percentage, total viable cell count, and median live cell diameter; and
ii) identify the cell suspension as having therapeutic potential for skin regeneration based on a comparison between the value indicative of the at least one characteristic of the cell suspension and a predetermined threshold.
8 . The system of claim 7 , wherein the predetermined threshold relating to total cell count is at least about 550,000 cells/ml.
9 . The system of claim 7 , wherein the predetermined threshold relating to cell viability percentage is at least about 30% viability.
10 . The system of claim 7 , wherein the predetermined threshold relating to total viable cell count at least about 300,000 cells/ml.
11 . The system of claim 7 , wherein the predetermined threshold relating to median live cell diameter is at least about 9 μm live cell mean diameter.