CELL SUSPENSION COMPOSITION WITH THERAPEUTIC POTENTIAL AND RELATED METHODS AND SYSTEMS FOR IDENTIFYING SAME
Systems and methods for identifying a cell suspension with therapeutic potential for skin regeneration and related compositions are disclosed herein. In some variations, a method may include receiving a cell suspension that comprises a population of viable cells and non-viable cells, then measuring a value indicative of at least one characteristic of the cell suspension, such as but not limited to one or more of total cell count, total cell viability, cell viability percentage, and median live cell diameter. A cell suspension composition having therapeutic potential may comprise a cell suspension met certain thresholds relating to total cell count, total viable cell count, cell viability percentage, and median live cell diameter.
1 . A cell suspension, comprising:
a population of cells derived from freshly disaggregated tissue, comprising at least 550,000 total cells per milliliter, wherein the population of cells has at least 30% viability, wherein at least 300,000 cells are viable, and wherein the median live cell diameter of the viable cells is greater than or equal to 9 micrometers; and
an effective amount of buffer.
2 . The cell suspension of claim 1 , wherein the tissue comprises skin tissue.
3 . The cell suspension of claim 2 , wherein the population of cells comprises keratinocytes, melanocytes, and fibroblasts.
4 . The cell suspension of claim 1 , wherein the population of cells is obtained by a process comprising:
obtaining a split-thickness skin sample;
contacting the epidermal sample with an effective amount of warmed enzyme solution;
contacting the epidermal sample with an effective amount of buffer;
mechanically disaggregating the epidermal sample;
contacting the epidermal sample with a second effective amount of buffer to create a buffered disaggregated tissue solution; and
passing the buffered disaggregated tissue solution through a filter, wherein the screen is at least as narrow as 100 micrometers.
6 . A cell suspension therapeutic, comprising:
a population of cells derived from freshly disaggregated tissue, comprising at least 550,000 total cells per milliliter, wherein the population of cells has at least 30% viability, wherein at least 300,000 cells are viable, and wherein the median live cell diameter of the viable cells is greater than or equal to 9 micrometers;
an effective amount of buffer; and
at least one scaffolding element.