ANTIOXIDANT POLYMERIC BIOMATERIAL FOR TISSUE ENGINEERING AND METHODS OF USING SAME
Provided are thiol-ene polymer networks which can reduce the ROS species that contribute to delayed bone healing and fusion. Furthermore, patients that suffer from neuropathic comorbidities such as diabetes suffer from a diminished healing capacity. An increase in proinflammatory factors and the high presence of reactive oxygen species (ROS) present in diabetics are linked to lower fusion rates. To this end, there is a need for a clinically relevant bone graft to promote bone fusions in patients with neuropathic comorbidities. Incorporating thiol-ene networks for bone scaffolds has demonstrated increased osteogenic biomarkers over traditional polymeric materials and act as antioxidants. Thiol-ene networks offer improved bone grafts for diabetic patients by reducing the number of hydroxyl radicals associated with neuropathic comorbidities. These networks are particularly well suited in promoting healing in patients with Type II Diabetes or other conditions exacerbated by ROS-mediated damage.
1 . A method of performing arthrodesis in an individual in need thereof, comprising:
providing an artificial bone graft, said bone graft comprising an effective amount of a methacrylate monomer and an effective amount of a thiol-containing macromer; and
implanting said artificial bone graft;
wherein said bone graft possesses antioxidant properties; and
wherein the bone graft scavenges reactive oxygen species and reduces osteoclastic activity at the fusion site.
2 . The method of claim 1 , wherein said individual is a Type II diabetic.
3 . The method of claim 1 , wherein said individual is osteoporotic.
4 . The method of claim 1 , wherein said arthrodesis is an MPJ fusion procedure.
5 . The method of claim 1 , wherein said methacrylate monomer is 1, 4-budanediol-dimethacrylate, diurethane dimethacrylate, or combinations thereof.
6 . The method of claim 1 , wherein said thiol-containing macromer is a multi-functional mercaptopropionate, mercaptoacetate, or combinations thereof.
7 . The method of claim 1 , wherein the methacrylate monomer and thiol-containing macromer are present at a ratio of 50:50.
8 . The method of claim 1 , wherein the methacrylate monomer and thiol-containing macromer are present at a ratio of 60:40.
9 . The method of claim 1 , wherein the methacrylate monomer and thiol-containing macromer are present at a ratio of 70:30.
10 . An oxidatively responsive polymeric scaffold for promoting bone fusion, comprising:
an effective amount of a methacrylate monomer; and
an effective amount of a thiol-containing macromer;
wherein said polymeric scaffold comprises sulfide linkages configured to sequester reactive oxygen species molecules; and
wherein said scaffold has a degradation rate which is complementary to the rate of bone healing.
11 . The scaffold of claim 10 , wherein said methacrylate monomer is 1,4-butanediol dimethacrylate.
12 . The scaffold of claim 10 , wherein said methacrylate monomer is diurethane dimethacrylate.
13 . The scaffold of claim 10 , wherein said thiol-containing macromer is a multi-functional mercaptopropionate, mercaptoacetate, or combinations thereof.
14 . The scaffold of claim 10 , further comprising a bioactive agent coupled to a thiolated group.
15 . The scaffold of claim 14 , wherein said bioactive agent is an Arginine-Glycine-Aspartate peptide sequence.
16 . The scaffold of claim 14 , wherein said bioactive agent is a TGF-β.
17 . The scaffold of claim 10 , wherein the methacrylate monomer and thiol-containing macromer are present at a ratio of 50:50.
18 . The scaffold of claim 10 , wherein the methacrylate monomer and thiol-containing macromer are present at a ratio of 70:30.
19 . The scaffold of claim 10 , wherein the methacrylate monomer and the thiol-containing macromer are present at a ratio of 60:40.
20 . The scaffold of claim 10 , wherein said scaffold is formulated as a bone graft.
21 . The scaffold of claim 10 , wherein said scaffold is formulated as an injectable gel.
22 . The scaffold of claim 10 , wherein said scaffold is formulated as an artificial joint surface comprising a hydrophilic layer within a matrix of the polymer.
23 . The scaffold of claim 10 , further comprising a plurality of pores having an average pore size between about 80 μm and 300 μm.