Methods and systems to collect a biological sample
Sample processing methods and systems to collect a biological sample. A device may be configured collect a predetermined volume of a sample in sample chamber and seal the chamber upon activation. The device may be further configured to mix the mix the sample with a predetermined volume of a reagent and/or mix the sample and the reagent in a pre-determined ratio.
1. A method of collecting a biological sample, the method comprising:
a. providing a device comprising:
i. a housing configurable from a first position to a second position;
ii. a collection well;
iii. a conduit comprising a capillary tube comprising openings at a first end and a second end, with the opening at the first end configured to receive the biological sample from the collection well;
iv. a sample storage chamber;
v. a mechanical actuator; and
vi. a reagent chamber within the housing comprising a fluid outlet and a fluid comprising one or more reagents within the reagent chamber and separated from the collection well and the sample storage chamber;
b. inserting a biological sample into the collection well;
c. transitioning the housing from the first position to the second position, thereby actuating the device and moving the biological sample into the sample storage chamber and moving the fluid comprising one or more reagents out of the reagent chamber via the fluid outlet;
d. storing the biological sample on a membrane; and
e. testing the biological sample on a test region of the membrane.
2. The method of claim 1 , further comprising moving the biological sample from the collection well into the conduit.
3. The method of claim 2 , comprising moving the biological sample from the collection well into the conduit by capillary action.
4. The method of claim 2 , further comprising moving the biological sample from the collection well into the sample storage chamber by a plunger activated by transitioning the housing from the first position to the second position.
5. The method of claim 1 , further comprising treating the biological sample with the one or more reagents.
6. The method of claim 5 , wherein the membrane is located within the sample storage chamber.
7. The method of claim 1 , wherein the membrane is in fluidic communication with the conduit.
8. The method of claim 1 , comprising transitioning the housing from the first position to the second position by pressing a first portion and a second portion of the housing together.
9. The method of claim 1 , further comprising dispensing the one or more reagents onto the membrane when transitioning the housing from the first position to the second position.
10. The method of claim 9 , further comprising treating the biological sample with the one or more reagents upon contacting the membrane.
11. The method of claim 1 , further comprising drying the biological sample.
12. The method of claim 1 , wherein the device further comprises a desiccant, the method further comprising drying the biological sample by removing moisture from the biological sample with the desiccant.
13. The method of claim 1 , wherein the housing defines an isolated internal air space in the second position.
14. The method of claim 1 , wherein the conduit is visible to a user when the housing is in the first position.
15. The method of claim 14 , further comprising verifying the movement of the biological sample from the collection well to the conduit by viewing the biological sample in the conduit prior to transitioning the housing from the first position to the second position.
16. The method of claim 1 , further comprising locking the housing in the second position upon transitioning the housing from the first position to the second position.
17. The method of claim 1 , further comprising transporting the device to a remote facility for processing of the biological sample.