SARS-COV-2 SPIKE PROTEIN ANTIBODIES
The present disclosure provides antibodies that bind to the SARS-CoV-2 spike protein, as well as compositions containing the same, and methods of making and using such a composition for treating, preventing, and/or detecting SARS-CoV-2 infection.
1 . A monoclonal antibody, or antigen binding portion thereof, that binds to SARS-Cov-2 spike protein (CoV-S), wherein the antibody comprises the vhCDR1, vhCDR2, vhCDR3, vlCDR1, vlCDR2 and vlCDR3 from an antibody selected from the group consisting of clone IDs: 3-P7-A ( FIG. 12H ), 5-P24-A ( FIG. 12N ), 9-K4-A ( FIG. 12R ), 10-B13-A ( FIG. 12V ), 10-L12-A ( FIG. 12W ), and 10-O24-A ( FIG. 12Y ).
2 . The monoclonal antibody, or antigen binding portion thereof, according to claim 1 , which comprises:
(i) the vhCDR1, vhCDR2, vhCDR3, vlCDR1, vlCDR2 and vlCDR3 sequences of SEQ ID NOS:76-81;
(ii) the vhCDR1, vhCDR2, vhCDR3, vlCDR1, vlCDR2 and vlCDR3 sequences of SEQ ID NOS:136-141;
(iii) the vhCDR1, vhCDR2, vhCDR3, vlCDR1, vlCDR2 and vlCDR3 sequences of SEQ ID NOS:176-181;
(iv) the vhCDR1, vhCDR2, vhCDR3, vlCDR1, vlCDR2 and vlCDR3 sequences of SEQ ID NOS:216-221;
(v) the vhCDR1, vhCDR2, vhCDR3, vlCDR1, vlCDR2 and vlCDR3 sequences of SEQ ID NOS:226-231; or
(vi) the vhCDR1, vhCDR2, vhCDR3, vlCDR1, vlCDR2 and vlCDR3 sequences of SEQ ID NOS:246-251.
3 . The monoclonal antibody, or antigen binding portion thereof, according to claim 1 , which comprises:
(i) the variable heavy domain (VH) and variable light domain (VL) according to SEQ ID NOS: 72 and 74;
(ii) the VH and VL according to SEQ ID NOS: 132 and 134;
(iii) the VH and VL according to SEQ ID NOS: 172 and 174;
(iv) the VH and VL according to SEQ ID NOS: 212 and 214;
(v) the VH and VL according to SEQ ID NOS: 222 and 224; or
(vi) the VH and VL according to SEQ ID NOS: 242 and 244.
4 . The monoclonal antibody according to claim 1 , wherein the monoclonal antibody is humanized.
5 . The monoclonal antibody according to claim 1 , wherein the monoclonal antibody is IgG1, IgG2, IgG3, or IgG4 class.
6 . The antigen binding portion of the monoclonal antibody according to claim 1 .
7 . The antigen binding portion according to claim 6 , wherein the antigen binding portion comprises a Fab fragment, Fv fragment, or single-chain Fv antibody.
8 . A composition comprising a monoclonal antibody, or antigen binding portion thereof, according to claim 1 .
9 . The composition according to claim 8 , wherein the monoclonal antibody, or antigen binding portion thereof, comprises the vhCDR1, vhCDR2, vhCDR3, vlCDR1, vlCDR2 and vlCDR3 sequences of: (i) SEQ ID NOS:76-81, (ii) SEQ ID NOS:136-141, (iii) SEQ ID NOS:176-181, (iv) SEQ ID NOS:216-221, (v) SEQ ID NOS:226-231, or (vi) SEQ ID NOS:246-251.
10 . The composition according to claim 8 , wherein the monoclonal antibody, or antigen binding portion thereof, comprises (i) the VH and VL domains according to SEQ ID NOS: 72 and 74, (ii) the VH and VL domains according to SEQ ID NOS: 132 and 134;
(iii) the VH and VL domains according to SEQ ID NOS: 172 and 174; (iv) the VH and VL domains according to SEQ ID NOS: 212 and 214; (v) the VH and VL domains according to SEQ ID NOS: 222 and 224; or (vi) the VH and VL domains according to SEQ ID NOS: 242 and 244.
11 . A cell line that expresses the monoclonal antibody, or antigen binding portion thereof, according to claim 1 .
12 . A composition comprising:
a first nucleic acid encoding the variable heavy domain (VH) of the antibody of claim 1 , and/or
a second nucleic acid encoding the variable light domain (VL) of the same antibody.
13 . The composition according to claim 12 , wherein the first nucleic acid molecule encodes the vhCDR1, vhCDR2, and vhCDR3 sequences of: (i) SEQ ID NOS:76-78, (ii) SEQ ID NOS:136-138, (iii) SEQ ID NOS:176-178, (iv) SEQ ID NOS:216-218, (v) SEQ ID NOS:226-228, or (vi) SEQ ID NOS:246-248.
14 . The composition according to claim 12 , wherein the second nucleic acid molecule encodes the vlCDR1, vlCDR2 and vlCDR3 sequences of: (i) SEQ ID NOS:79-81, (ii) SEQ ID NOS:139-141, (iii) SEQ ID NOS:179-181, (iv) SEQ ID NOS:219-221, (v) SEQ ID NOS:229-231, or (vi) SEQ ID NOS:249-251.
15 . The composition according to claim 12 , wherein the first nucleic acid molecule encodes the VH domain comprising the amino acid sequence of one of SEQ ID NOS: 72, 132, 172, 212, 222, and 242 . and/or
the second nucleic acid molecule encodes the VL domain comprising the amino acid sequence of one of SEQ ID NOS: 74, 134, 174, 214, 224, and 244.
16 . (canceled)
17 . An expression vector comprising the first and second nucleic acids of claim 12 .
18 . A host cell comprising the expression vector of claim 17 .
19 . A method of making an antibody, or antigen binding portion thereof, comprising:
culturing the host cell of claim 18 under conditions wherein the antibody, or antigen binding portion thereof, is produced; and
recovering the antibody or antigen binding portion thereof.
20 . A method of treating or preventing SARS-CoV-2 infection in a patient in need comprising administering to the patient the monoclonal antibody, or antigen binding portion thereof, according to claim 1 .
21 . A method of detecting SARS-CoV-2 in a human sample comprising:
contacting the human sample with the monoclonal antibody, or antigen binding portion thereof, according to claim 1 , and
detecting binding of the antibody, or antigen binding portions thereof, to SARS-CoV-2 spike protein (CoV-S) as an indication of presence of SARS-CoV-2 in the sample.