METHODS AND COMPOSITIONS FOR DETECTING GENETIC MATERIAL
This invention provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some cases, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.
1 . A method of detecting a fetal genetic condition comprising:
a. obtaining a mixture of maternal and fetal genetic material comprising target polynucleotides;
b. combining said mixture with targeting oligonucleotides that bind said target polynucleotides;
c. subdividing said targeting oligonucleotides into reaction volumes, wherein at least one of said reaction volumes comprises no said target polynucleotide and no said targeting oligonucleotide;
d. performing an amplification reaction within said reaction volumes;
e. detecting a presence of said target polynucleotide or said targeting oligonucleotide within said reaction volumes; and
f. determining the relative level of said target polynucleotide in said mixture in order to detect a fetal genetic condition.
2 . The method of claim 1 , wherein said reaction volumes are selected from the group consisting of wells and aqueous droplets within a continuous oil phase.
3 . The method of claim 1 , wherein said targeting oligonucleotides are selected from the group consisting of primer pairs, ligation probes, molecular inversion probes, and padlock probes.
4 . The method of claim 1 , the target polynucleotides comprising a genetic target, wherein said targeting oligonucleotides include a pair of targeting oligonucleotides designed to recognize neighboring regions of the genetic target.
5 . The method of claim 1 , the target polynucleotides comprising a genetic target, wherein said targeting oligonucleotides include multiple pairs of targeting oligonucleotides designed to recognize different portions of said genetic target.
6 . The method of claim 4 , the target polynucleotides further comprising a second genetic target, wherein said targeting oligonucleotides include a different pair of targeting oligonucleotides designed to recognize neighboring regions of the second genetic target.
7 . The method of claim 6 , wherein half the targeting oligonucleotides recognize a suspected aneuploid chromosome, while the other half recognize a presumed diploid chromosome.
8 . The method of claim 1 , the target polynucleotides comprising first and second genetic targets, wherein two different colors are used to detect the two different genetic targets.
9 . The method of claim 1 , wherein said target polynucleotide is within a chromosome selected from the group consisting of chromosome 13, 18, 21, and X.
10 . The method of claim 1 , wherein said determining in step (f) comprises comparing a number of reaction volumes comprising an amplified product of said targeting oligonucleotides with a number of reaction volumes comprising an amplified product of targeting oligonucleotides directed to a reference polynucleotide.
11 . A method of detecting copy number of a target polynucleotide within a population of genetic material comprising:
a. binding a first ligation probe to a first target polynucleotide;
b. binding a second ligation probe to a second target polynucleotide;
c. subjecting said first and second ligation probes to a ligation reaction to obtain one or more ligated products;
d. partitioning said one or more ligated products into two or more partitions;
e. amplifying a region within said one or more ligated products to obtain amplified products;
f. determining a number of said partitions that contain said amplified products; and
g. calculating a copy number of said first target polynucleotide based on said number of said partitions.
12 . The method of claim 10 , wherein the partitions are selected from the group consisting of wells and aqueous droplets within a continuous oil phase.
13 . The method of claim 1 , further comprising a third ligation probe, wherein said first ligation probe is designed to bind to a first region within said first target polynucleotide and said third ligation probe is designed to bind to a second region within said first target polynucleotide, wherein said first and second regions of said first target polynucleotide do not have identical sequences.
14 . The method of claim 13 , further comprising a fourth ligation probe, wherein said second ligation probe is designed to bind to a first region within said second target polynucleotide and said fourth ligation probe is designed to bind to a second region within said second target polynucleotide, wherein said first and second regions of the second target polynucleotide do not have identical sequences.
15 . The method of claim 13 , further comprising a fifth ligation probe, wherein said fifth ligation probe is designed to bind to a third region within said first target polynucleotide, wherein said first, second, and third regions of said first target polynucleotide do not have identical sequences.
16 . The method of claim 1 , further comprising a collection of probes to recognize different regions of said first target polynucleotide.
17 . The method of claim 16 , further comprising a collection of probes to recognize different regions of said second target polynucleotide.
18 . The method of claim 11 , wherein said first target polynucleotide is a test chromosome and said second target polynucleotide is a reference chromosome.
19 . The method of claim 1 , wherein said first target polynucleotide is a segment of a chromosome.
20 . The method of claim 1 , wherein said first ligation probe is conjugated to a first signaling agent and wherein said second ligation probe is conjugated a second signaling agent;
wherein said first signaling agent is a fluorescent marker of a first color and said second signaling agent is a fluorescent marker of a second color;
wherein said determining of step (f) comprises detecting said first ligation probe with a first signaling agent and detecting said second ligation probe with a second signaling agent; and
wherein said first ligation probe comprises a plurality of ligation probes, wherein each probe is directed to a different region of a first chromosome, and wherein said second ligation probe comprises a plurality of ligation probes, wherein each probe is directed to a different region of a second chromosome.