IP Library › Granted Patent US 12,227,773
Granted Patent B2
US 12,227,773 · App. 17/750,758 · Granted Feb 18, 2025

Recombinase polymerase amplification

Inventors: Olaf Piepenburg (Cambridge, GB); Niall A. Armes (Essex, GB); Matthew James David Parker (Hertfordshire, GB)
Assignee: ABBOTT DIAGNOSTICS SCARBOROUGH, INC.
C12N9/1241C12N9/00C12Q1/6844C12Y207/07
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Quick Facts
Patent No.
US 12,227,773
App. No.
17/750,758
Granted
Feb 18, 2025
Kind
B2
Abstract

The present invention features novel, diverse, hybrid and engineered recombinase enzymes, and the utility of such proteins with associated recombination factors for carrying out DNA amplification assays. The present invention also features different recombinase ‘systems’ having distinct biochemical activities in DNA amplification assays, and differing requirements for loading factors, single-stranded DNA binding proteins (SSBs), and the quantity of crowding agent employed.

Claims (9)

1. A composition comprising:

a mutant UvsX protein comprising an amino acid substitution of a histidine in a Walker A box amino acid sequence; and

a crowding agent.

2. The composition of claim 1 , wherein the amino acid substitution of the histidine is an amino acid substitution of a serine for the histidine in the Walker A box amino acid sequence.

3. The composition of claim 1 , wherein the Walker A box amino acid sequence comprises an amino acid sequence that is GPSKSFKS (SEQ ID NO: 46).

4. A recombinase polymerase reaction mixture comprising the composition of claim 1 .

5. A nucleic acid encoding the mutant UvsX protein of claim 1 .

6. A vector comprising the nucleic acid of claim 5 .

7. A cell comprising the nucleic acid of claim 5 .

Assignments (5)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 25, 2022
From: PIEPENBURG, OLAF; ARMES, NIALL A.; PARKER, MATHEW JAMES DAVID
To: ASM SCIENTIFIC, INC.
Reel/Frame 060897/0753 →
MERGER Recorded Aug 25, 2022
From: TWISTDX, INC.
To: BIOSITE INCORPORATED
Reel/Frame 060897/0777 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 25, 2022
From: ALERE SAN DIEGO INC.
To: ABBOTT DIAGNOSTICS SCARBOROUGH, INC.
Reel/Frame 060897/0795 →
CHANGE OF NAME Recorded Aug 25, 2022
From: BIOSITE INCORPORATED
To: ALERE SAN DIEGO INC.
Reel/Frame 061316/0407 →
CHANGE OF NAME Recorded Aug 25, 2022
From: ASM SCIENTIFIC, INC.
To: TWISTDX, INC.
Reel/Frame 061327/0295 →
Continuity (6)
Continuation 16155133 · Oct 9, 2018
Continuation 14135227 · Dec 19, 2013
Continuation 13307293 · Nov 30, 2011
Continuation 11800318 · May 4, 2007
Provisional Application 60798060 · May 4, 2006
Related Publication 20230242889A1 · Aug 3, 2023
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