Loop-mediated isothermal amplification primers for vibrio parahaemolyticus detection and uses thereof
Primers and primer sets for amplification of Vibrio parahaemolyticus DNA and methods of detecting Vibrio parahaemolyticus using the primers and primer sets.
1 . A primer set comprising a primer having at least 90% homology over the full length to SEQ ID NO: 1, and optionally comprising a primer having at least 80% homology over the full length to SEQ ID NO: 4.
2 . The primer set of claim 1 , comprising a primer having at least 95% homology over the full length to SEQ ID NO: 1.
3 . The primer set of claim 1 , comprising a primer having SEQ ID NO: 1.
4 . The primer set of claim 1 , comprising a primer having at least 95% homology to SEQ ID NO: 4.
5 . The primer set of claim 1 , comprising a primer having SEQ ID NO: 4.
6 . The primer set of claim 1 , further comprising a primer having at least 80% homology to SEQ ID NO: 2.
7 . The primer set of claim 1 , comprising a primer having SEQ ID NO: 2.
8 . The primer set of claim 1 , further comprising a primer having least 80% homology to SEQ ID NO: 3.
9 . The primer set of claim 1 , comprising a primer having SEQ ID NO: 3.
10 . The primer set of any of claim 1 , further comprising a primer having least 80% homology to SEQ ID NO: 5.
11 . The primer set of claim 1 , comprising a primer having SEQ ID NO: 5.
12 . The primer set of claim 1 , further comprising a primer having at least 80% homology to SEQ ID NO: 6.
13 . The primer set of claim 1 , comprising a primer having SEQ ID NO: 6.
14 . A lyophilized primer set comprising the primer set of claim 1 .
15 . A pellet comprising the lyophilized primer set of claim 14 .
16 . A method of amplifying target DNA comprising exposing the target DNA to the primer set of claim 1 under conditions that are suitable for amplification of the target DNA.
17 . The method of claim 16 , wherein the conditions include a temperature of 50° C. to 70° C.
18 . The method of claim 16 , further comprising detecting the presence of amplified target DNA.
19 . The method of claim 16 , wherein the target DNA is DNA of V. parahaemolyticus.
20 . The method of claim 16 , wherein the target DNA is DNA of the VP175 gene of V. parahaemolyticus.