Granted Patent B2

US 12,606,588 · App. 17/764,874 · Granted Apr 21, 2026

Inventors: Tracy Matray (Snohomish, WA); Erin Kwang (New York, NY); Melissa Dominguez (New York, NY)

Assignee: SONY GROUP CORPORATION

C07H21/00C12Q1/6816C12Q1/6876

Abstract

Claims (28)

1 . A compound having the following structure (I):

or a stereoisomer, salt or tautomer thereof, wherein:

M is, at each occurrence, independently either: a) the same or different fluorophore; or b) the same or different fluorophore quencher;

L 1a is, at each occurrence, independently a heteroarylene linker;

L 2 and L 8 are independently linkers or absent;

L 1b , L 3 , L 5 , L 6 and L 7 are, at each occurrence, independently absent, or alkylene, alkenylene, alkynylene, heteroalkylene, heteroalkenylene or heteroalkynylene linkers;

L 4 is, at each occurrence, independently an alkylene, alkenylene, alkynylene, heteroalkylene, heteroalkenylene or heteroalkynylene linker;

R 1 and R 2 each comprise a polynucleotide or —OP(═R a )(R b )R c , provided that at least one of R 1 and R 2 comprises a polynucleotide;

R 3 is, at each occurrence, independently H, alkyl or alkoxy;

R 4 is, at each occurrence, independently OH, SH, O − , S − , OR d or SR d ;

R 5 is, at each occurrence, independently oxo, thioxo or absent;

R a is O or S;

R b is OH, SH, O − , S − , OR d or SR d ;

R c is OH, SH, O − , S − , OR d , OL′, SR d , alkyl, alkoxy, heteroalkyl, heteroalkoxy, alkylether, alkoxyalkylether, phosphate, thiophosphate, phosphoalkyl, thiophosphoalkyl, phosphoalkylether or thiophosphoalkylether;

R d is a counter ion;

L′ is, at each occurrence, independently a linker comprising a covalent bond to a solid support, a linker comprising a covalent bond to a solid support residue or a linker comprising a covalent bond to a nucleoside;

m is, at each occurrence, independently an integer of zero or greater;

n is an integer from 2 to 10; and

q and w are, at each occurrence, independently 0 or 1, provided that at least one occurrence of either q or w is 1.

2 . The compound of claim 1 , wherein the compound has one of the following structures:

wherein:

has the following structure

has the following structure

dT has the following structure

DAB has the following structure

F has the following structure

and

BHQ1 has the following structure

Assignments (3)

Continuity (2)

References Cited (400)