METHOD TO INCREASE ANTIBODY YIELD DURING ION EXCHANGE CHROMATOGRAPHY
The present invention relates to a method for increasing antibody yield during antibody purification from a sample by ion exchange chromatography in flow-through mode by pre-conditioning the sample with Tris without the use of NaCl to adjust the conductivity.
1 . A method for increasing the yield of an antibody in the flow through of ion exchange chromatography during antibody purification comprising the steps of:
a. providing a sample comprising an antibody;
b. adjusting the conductivity of the sample;
c. processing the adjusted sample by ion exchange chromatography in flow-through mode and
d. collecting the flow-through comprising the antibody,
wherein the conductivity of the sample in step b) is adjusted with Tris to at least 10 mS/cm and wherein the pH after adjusting the conductivity is in the range of pH 6.5 to 7.5.
2 . The method according to claim 1 , wherein the sample comprising an antibody is an affinity chromatography eluate.
3 . The method according claim 2 , wherein the affinity chromatography eluate is a Protein A chromatography eluate with a pH of about 3 to about 4.
4 . The method according to claim 3 , wherein the pH of about 3 to about 4 of the sample is adjusted to a pH of about 5.2 to about 5.6, preferably to a pH of 5.5.
5 . The method according to claim 1 , wherein the conductivity of the sample is adjusted to a conductivity between 10 and 50 mS/cm.
6 . The method according to claim 5 , wherein the conductivity is adjusted to the range of 13 to 30 mS/cm.
7 . The method according to claim 5 , wherein the conductivity is adjusted to 15 mS/cm.
8 . The method according to claim 1 , wherein the ion exchange chromatography is multimodal anion exchange chromatography.
9 . The method according to claim 1 , wherein the monoclonal antibody to be purified is a monoclonal antibody.
10 . The method according to claim 9 , wherein the monoclonal antibody to be purified is an anti-IL17c antibody.
11 . The method according to claim 10 , wherein the monoclonal anti-IL17C antibody to be purified comprises a VH of SEQ ID NO: 8 and a VL of SEQ ID NO: 7.
12 . The method according to claim 11 , wherein the monoclonal anti-IL17C antibody to be purified consists of a heavy chain of SEQ ID NO:10 and a light chain of SEQ ID NO: 9.
13 . The method according to claim 1 , wherein the yield of the purified antibody in the flow-through is more than 75%.
14 . The method according to claim 1 , wherein the conductivity of the sample in step b) is adjusted with Tris in the absence of NaCl.
15 . A pharmaceutical composition obtained by the method according to claim 1 .