BUOYANT HOLLOW PARTICLES COMPOSTION AND METHOD
A composition and a method for culturing cells. The composition includes a plurality of buoyant hollow particles, the buoyant hollow particles comprising a siliceous surface; and a plurality of mammalian cells attached to the siliceous surface of the buoyant hollow particles; wherein the buoyant hollow particles are less dense than a media; and wherein the average seeding density is 3-50 adherent cells/buoyant hollow particle.
1 . A composition comprising
a plurality of buoyant hollow particles, the buoyant hollow particles comprising a siliceous surface; and
a plurality of mammalian cells attached to the siliceous surface of the buoyant hollow particles;
wherein the buoyant hollow particles are less dense than a media; and
wherein the average seeding density is 3-50 adherent cells/buoyant hollow particle.
2 . The composition according to claim 1 , wherein the buoyant hollow particles comprise glass bubbles.
3 . The composition according to claim 1 , wherein the media comprises water.
4 . The composition according to claim 1 , wherein the siliceous surface of the buoyant hollow particles are functionalized, coated or treated to provide a surface for growth of mammalian cells.
5 . The composition according to claim 1 , wherein the siliceous surface of the buoyant hollow particles are coated with a polymer, a signaling peptide or an antibody or receptor, a surface-modifying agent or a substrate.
6 . The composition according to claim 5 , wherein the surface-modifying agent is bonded to the buoyant hollow particles.
7 . The composition according to claim 1 , wherein the buoyant hollow particles have a mean particle size of from 10 μm to 120 μm.
8 . The composition according to claim 1 , wherein the buoyant hollow particles have an average density of less than about 1 g/ml.
9 . A method for culturing cells, the method comprising providing a plurality of buoyant hollow particles, the buoyant hollow particles comprising a siliceous surface, wherein the buoyant hollow particles are less dense than a media;
contacting the buoyant hollow particles with a media comprising a plurality of mammalian cells;
allowing the mammalian cells to attach to the siliceous surface of the buoyant hollow particles; and
culturing the mammalian cells on the siliceous surface of the buoyant hollow particles by agitation, to yield the cell culture.
10 . The method according to claim 9 , further comprising separating the buoyant hollow particles with the mammalian cells attached from the media.
11 . The method according to claim 10 , wherein the separating step comprises allowing the buoyant hollow particles with the mammalian cells attached to float to an upper surface of the media.
12 . The method according to claim 9 , further comprising detaching the mammalian cells from the siliceous surface of the buoyant hollow particles.
13 . The method according to claim 9 , further collecting the detached mammalian cells by sedimentation or centrifugation.
14 . The method according to claim 9 , further comprising adding additional cell culture media or additional buoyant hollow particles.
15 . The method according to claim 9 , where in the average seeding density is 3-50 adherent cells/buoyant hollow particle.