Oblique plane microscope and method for correcting an aberration in an oblique plane microscope
An oblique plane microscope includes an optical imaging system configured to form an image of an object. The optical imaging system includes a telescope system with an optical zoom system, which is adjustable for adapting a magnification of the telescope system to a ratio between two refractive indices, one of which being associated with an object side of the telescope system and the other being associated with an image side of the telescope system. The oblique plane microscope further includes a control unit. The control unit is configured to evaluate an image quality of the image formed by the optical imaging system and to adjust the optical zoom system based on the evaluation.
1. An oblique plane microscope, comprising:
an optical imaging system configured to form an image of an object, the optical imaging system comprising a telescope system with an optical zoom system, which is adjustable for adapting a magnification of the telescope system to a ratio between two refractive indices, one of which being associated with an object side of the telescope system and the other being associated with an image side of the telescope system; and
a control unit,
wherein the telescope system comprises an objective arranged at the object side of the telescope system, wherein the control unit is configured to divide the image formed by the oblique plane microscope into three or more areas, wherein a first area comprises an image of a first region of the object and a second area comprises an image of a second region of the object, wherein the first region is located off of a focal plane of the objective on a first side facing away from the objective, and the second region is located off of the focal plane of the objective on a second side facing the objective, and wherein the control unit is configured to evaluate an image quality only in the first area and/or the second area, and to adjust the optical zoom system based on the evaluation.
2. The oblique plane microscope according to claim 1 , wherein a third area comprises an image of a third region of the object, the third region being intersected by the focal plane of the objective.
3. The oblique plane microscope according to claim 2 , wherein the telescope system comprises an optical correction apparatus which is adjustable for correcting a spherical aberration of the optical imaging system, and wherein the control unit is configured to evaluate an image quality of the third area of the image and to adjust the optical correction apparatus based on the evaluation of the image quality in the third area.
4. The oblique plane microscope according to claim 3 , wherein the control unit is configured to evaluate the image quality of the first area and/or the second area, to evaluate the image quality in the third area, and to adjust the optical correction apparatus and the optical zoom system based on the evaluation of the image quality in the first area and/or the second area and the evaluation of the image quality in the third area in an iterative process.
5. The oblique plane microscope according to claim 1 , wherein the control unit is configured to evaluate the image quality by determining a Strehl ratio, a contrast value, an image sharpness measure and/or a width of an autocorrelation function of the image.
6. The oblique plane microscope according to claim 1 , wherein the optical zoom system is configured to render the telescope system telecentric contained over an entire magnification range with respect to both the object side and the image side.
7. The oblique plane microscope according to claim 6 , wherein the magnification range of the telescope system corresponds to a range in which the ratio of the two refractive indices is between 1.0 and 1.6.
8. The oblique plane microscope according to claim 1 , wherein the telescope system is formed by a Keplerian telescope comprising the optical zoom system.
9. A method for correcting an aberration in an oblique plane microscope, the method comprising:
evaluating, by a control unit of the oblique plane microscope, an image quality of an image of an object formed by an optical imaging system of the oblique plane microscope, wherein the optical imaging system comprises a telescope system with an optical zoom system, which is adjustable for adapting a magnification of the telescope system to a ratio between two refractive indices, one of which being associated with an object side of the telescope system and the other being associated with an image side of the telescope system, wherein the telescope system comprises an objective arranged at the object side of the telescope system, wherein the image comprises three or more areas, a first area comprising an image of a first region of the object, and a second area comprising an image of a second region of the object, wherein the first region is located off of a focal plane of the objective on a first side facing away from the objective, and the second region is located off of the focal plane of the objective on a second side facing the objective, wherein the evaluating the image quality comprises evaluating the image quality only in the first area and/or the second area, and
adjusting, by the control unit, the optical zoom system of the oblique plane microscope based on the evaluation of the image quality only in the first area and/or the second area.
10. The method according to claim 9 , wherein the telescope system comprises an optical correction apparatus which is adjustable for correcting a spherical aberration of the optical imaging system, wherein the image comprises a third area, the third area comprising an image of a third region of the object, the third region being intersected by the focal plane of the objective, the method further comprising:
evaluating, by the control unit, an image quality in the third area, and
adjusting, by the control unit, the optical correction apparatus based on the evaluation of the image quality in the third area.